Two Dimensional Electrophoresis

Author : Bonnie S. Dunbar
Publisher : Springer
ISBN 13 : 9780306428395
Total Pages : 398 pages
Book Rating : 4.93/5 ( download)

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Download or read book Two-Dimensional Electrophoresis and Immunological Techniques written by Bonnie S. Dunbar and published by Springer. This book was released on 1988-07-31 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is a summary of basic principles and techniques and is dedicated to all those students who have been told by their mentors, "Go forth and do two-dimensional gels and have the results on my desk tomorrow. " No attempt has been made in this text to provide exhaustive lists of references related to basic principles or techniques or to list every com pany or supplier involved in this area of research. Nevertheless, it is hoped that sufficient information is given to help a new investigator or student appreciate the complexities but develop sufficient expertise to carry out these techniques successfully. The discussions are designed to instill in basic science and clinical investigators of all levels of expertise an appreciation of the power of combining a variety of techniques as well as to provide basic insight into the theories, complexities, and problems frequently encountered with electrophoretic and immunochemical meth ods. Bonnie S. Dunbar Houston v Acknowledgments I wish to thank my students and staff for their patience and support throughout the preparation of this text. I would like to acknowledge my appreciation for my extensive discussions with Dr. David Sammons (Uni versity of Arizona) and to Dr. N. L. and Dr. N. G. Anderson and their colleagues (Argonne National Laboratory) for their invaluable advice and suggestions in this area over the years. I thank my research assistant, Ms.

Author : T. Rabilloud
Publisher : Springer Science & Business Media
ISBN 13 : 9783540657927
Total Pages : 272 pages
Book Rating : 4.24/5 ( download)

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Download or read book Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods written by T. Rabilloud and published by Springer Science & Business Media. This book was released on 1999-09-17 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.

Author : Julio Celis
Publisher : Elsevier
ISBN 13 : 0323147917
Total Pages : 514 pages
Book Rating : 4.10/5 ( download)

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Download or read book Two-Dimensional Gel Electrophoresis of Proteins written by Julio Celis and published by Elsevier. This book was released on 2012-12-02 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications reviews current methods and clinical applications of two-dimensional gel electrophoresis of proteins, including the QUEST system, silver staining, and peptide mapping. Two-dimensional gel electrophoresis are applied to the study of diseases ranging from inborn errors of metabolism to human germ-line mutation rates, cancer, and mistranslation in animal and bacterial cells. This volume is organized into three sections encompassing 14 chapters and begins with an overview of the methodology of two-dimensional gel electrophoresis, followed by a discussion of computerized two-dimensional gel electrophoresis, silver staining, immunoblotting, and one- and two-dimensional peptide mapping. In most cases, a step-by-step guide to the techniques is given so that procedures may be easily repeated. A catalog of mouse fibroblast proteins is also given. The chapters that follow focus mainly on applications of two-dimensional gel electrophoresis in areas such as clinical and cancer research, human genetics, protein biosynthesis, and gene expression in plants. The final section presents current protein catalogs of Escherichia coli and human HeLa cells. This book is suitable for young researchers as well as for senior scientists working with a wide variety of problems in molecular and cell biology, basic biochemistry, genetics, and clinical research.

Author : David Sheehan
Publisher : Humana Press
ISBN 13 : 9781617378492
Total Pages : 0 pages
Book Rating : 4.96/5 ( download)

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Download or read book Two-Dimensional Electrophoresis Protocols written by David Sheehan and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human genome and other large-scale genome sequencing projects have inevitably led to a focus on the proteins encoded by genes. The field of proteomics has grown enormously as a result and a number of high-throughput technologies have now been developed allowing discovery-led investigations of protein populations rather than more traditional hypothesis-led studies on single proteins. These high-throughput techno- gies include gene and protein microarrays, the yeast two-hybrid system, and various mass spectrometry methodologies. However, despite developments and improvements in these technologies, two-dimensional electrophoresis (2DE) remains one of the most widely used approaches. This technique was revolutionised by the development of immobilised pH gradient strips which are now commercially available. This has made possible highly reproducible separations of matched samples. Developments in staining, mass spectr- etry, and bioinformatics supported these developments and have led to a measure of standardisation in design, execution, and analysis of proteomics experiments. This book began life as a proposed update of the excellent volume 2DE Protocols edited by Andrew Link of the University of Washington at Seattle. However, we re- ised that 2DE has undergone major development in aspects of its technology in recent years and we were anxious to reflect these in the present volume. We are also conscious that many researchers have now begun to apply proteomics methodologies to a growing range of biological material and we were anxious to include contributions to reflect the challenges posed in sample preparation in less widely used organisms.

Author : T. Rabilloud
Publisher : Springer Science & Business Media
ISBN 13 : 3642571050
Total Pages : 256 pages
Book Rating : 4.53/5 ( download)

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Download or read book Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods written by T. Rabilloud and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.

Author : Oscar Alzate
Publisher : CRC Press
ISBN 13 : 1420076264
Total Pages : 356 pages
Book Rating : 4.64/5 ( download)

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Download or read book Neuroproteomics written by Oscar Alzate and published by CRC Press. This book was released on 2009-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg

Author : Andrew J. Link
Publisher : Springer Science & Business Media
ISBN 13 : 1592595847
Total Pages : 585 pages
Book Rating : 4.46/5 ( download)

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Download or read book 2-D Proteome Analysis Protocols written by Andrew J. Link and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

Author : Kay Ohlendieck
Publisher : Springer Nature
ISBN 13 : 1071628313
Total Pages : 477 pages
Book Rating : 4.17/5 ( download)

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Download or read book Difference Gel Electrophoresis written by Kay Ohlendieck and published by Springer Nature. This book was released on 2022-11-15 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Author : N Saraswathy
Publisher : Elsevier
ISBN 13 : 1908818050
Total Pages : 271 pages
Book Rating : 4.58/5 ( download)

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Download or read book Concepts and Techniques in Genomics and Proteomics written by N Saraswathy and published by Elsevier. This book was released on 2011-07-01 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concepts and techniques in genomics and proteomics covers the important concepts of high-throughput modern techniques used in the genomics and proteomics field. Each technique is explained with its underlying concepts, and simple line diagrams and flow charts are included to aid understanding and memory. A summary of key points precedes each chapter within the book, followed by detailed description in the subsections. Each subsection concludes with suggested relevant original references. Provides definitions for key concepts Case studies are included to illustrate ideas Important points to remember are noted

Author : A. Thomas Endler
Publisher : VCH Publishers
ISBN 13 :
Total Pages : 364 pages
Book Rating : 4.90/5 ( download)

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Download or read book Two-dimensional Electrophoresis written by A. Thomas Endler and published by VCH Publishers. This book was released on 1989 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: