Fluorescent Materials For Cell Imaging

Author : Fu-Gen Wu
Publisher : Springer Nature
ISBN 13 : 981155062X
Total Pages : 247 pages
Book Rating : 4.21/5 ( download)

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Download or read book Fluorescent Materials for Cell Imaging written by Fu-Gen Wu and published by Springer Nature. This book was released on 2020-10-26 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the latest fluorescent materials for cell imaging. Cell imaging is a widely used basic technique that helps scientists gain a better understanding of biological functions through studies of cellular structure and dynamics. In the past decades, the development of a variety of new fluorescent materials has significantly extended the applications of cellular imaging techniques. This book presents recently developed fluorescent materials, including semiconductor quantum dots, carbon dots, silicon nanoparticles, metal nanoclusters, upconversion nanoparticles, conjugated polymers/polymer dots, aggregation-induced emission (AIE) probes, and coordination compounds, used for various cellular imaging purposes. It will appeal to cell biologists and other researchers in academia, industry and clinical settings who are interested in the technical development and advanced applications of fluorescence imaging in cells, tissues and organisms to explore the mechanisms of biological functions and diseases.

Author : Natalia Grigoryeva
Publisher : BoD – Books on Demand
ISBN 13 : 1839680393
Total Pages : 464 pages
Book Rating : 4.97/5 ( download)

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Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva and published by BoD – Books on Demand. This book was released on 2020-09 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Author : Vivek Mishra
Publisher : CRC Press
ISBN 13 : 1040036775
Total Pages : 347 pages
Book Rating : 4.78/5 ( download)

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Download or read book Functional Fluorescent Materials written by Vivek Mishra and published by CRC Press. This book was released on 2024-06-17 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: Functional Fluorescent Materials: Applications in Sensing, Bioimaging, and Optoelectronics explains functional molecular probes (organic/inorganic materials, polymers, nanomaterials), with a focus on those that represent spectroscopic properties with detection of different analytes and specific roles in molecular recognition and their applications. It broadly covers molecular recognition to applications of fluorescence reporters, starting from optoelectronic properties of materials, detection of heavy metals, through biological macromolecules, and further to a living cell, tissue imaging, and theranostics. Features: • Covers different aspects of fluorescence spectroscopy ranging from chemical, physical, and biological aspects along with optoelectronic properties, mechanisms, and applications. • Describes all types of chemical and functionalized fluorescent nanomaterials. • Provides additional information on different kinds of fluorescence reporters. • Explains the concept of fluorescence spectroscopy and its role in human health care. • Discusses changes in static and dynamic properties of fluorescent probes and molecular recognitions. This book is aimed at graduate students and researchers in materials, chemical engineering, and engineering physics.

Author : Vivek Mishra
Publisher :
ISBN 13 : 9781032402987
Total Pages : 0 pages
Book Rating : 4.89/5 ( download)

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Download or read book Functional Fluorescent Materials written by Vivek Mishra and published by . This book was released on 2024-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author :
Publisher : Academic Press
ISBN 13 : 9780080859286
Total Pages : 502 pages
Book Rating : 4.83/5 ( download)

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Download or read book Fluorescence Microscopy of Living Cells in Culture, Part B written by and published by Academic Press. This book was released on 1989-04-01 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy of Living Cells in Culture, Part B

Author : J. Slavík
Publisher : Springer Science & Business Media
ISBN 13 : 9780306453922
Total Pages : 338 pages
Book Rating : 4.24/5 ( download)

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Download or read book Fluorescence Microscopy and Fluorescent Probes written by J. Slavík and published by Springer Science & Business Media. This book was released on 1996-10-31 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
ISBN 13 : 3642180353
Total Pages : 352 pages
Book Rating : 4.54/5 ( download)

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Download or read book Advanced Fluorescence Reporters in Chemistry and Biology III written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2011-03-29 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.

Author : Alexander P. Demchenko
Publisher : Springer Nature
ISBN 13 : 3031190890
Total Pages : 772 pages
Book Rating : 4.96/5 ( download)

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Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Nature. This book was released on 2023-02-15 with total page 772 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. This book provides systematic knowledge of basic principles in design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Author : Ammasi Periasamy
Publisher : Springer
ISBN 13 : 1461475139
Total Pages : 473 pages
Book Rating : 4.32/5 ( download)

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Download or read book Methods in Cellular Imaging written by Ammasi Periasamy and published by Springer. This book was released on 2013-05-27 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Author : Alexander P. Demchenko
Publisher : Springer Nature
ISBN 13 : 3030601552
Total Pages : 673 pages
Book Rating : 4.53/5 ( download)

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Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Nature. This book was released on 2020-12-01 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.