Flow Cytometry And Cell Sorting

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
ISBN 13 : 3662041294
Total Pages : 365 pages
Book Rating : 4.91/5 ( download)

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Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
ISBN 13 : 3662027852
Total Pages : 220 pages
Book Rating : 4.51/5 ( download)

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Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of these procedures, with emphasis on immunofluorescence (antibody-fluorochrome conjugation, staining principles and data evaluation); the isolation of specific chromosomes, cells and fragile, large particles by magnetic and fluorescence-activated sorting; cellular biochemistry; and the dynamics of proliferation. The methods have been field-tested in recent EMBO courses on flow cytometry.

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
ISBN 13 : 9783540656302
Total Pages : 1366 pages
Book Rating : 4.08/5 ( download)

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Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2000 with total page 1366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Author : Marion G. Macey
Publisher : Springer Science & Business Media
ISBN 13 : 1597454516
Total Pages : 294 pages
Book Rating : 4.13/5 ( download)

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Download or read book Flow Cytometry written by Marion G. Macey and published by Springer Science & Business Media. This book was released on 2007-11-03 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Author : Howard M. Shapiro
Publisher : John Wiley & Sons
ISBN 13 : 0471434035
Total Pages : 736 pages
Book Rating : 4.30/5 ( download)

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Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Author : Rochelle A. Diamond
Publisher : Springer Science & Business Media
ISBN 13 : 3642570496
Total Pages : 799 pages
Book Rating : 4.90/5 ( download)

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Download or read book In Living Color written by Rochelle A. Diamond and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 799 pages. Available in PDF, EPUB and Kindle. Book excerpt: A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.

Author : Ashok Kumar
Publisher : Springer
ISBN 13 : 9783642094545
Total Pages : 0 pages
Book Rating : 4.46/5 ( download)

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Download or read book Cell Separation written by Ashok Kumar and published by Springer. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts

Author : Teresa S. Hawley
Publisher : Springer Nature
ISBN 13 : 107163738X
Total Pages : 460 pages
Book Rating : 4.88/5 ( download)

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Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Alice Longobardi Givan
Publisher : John Wiley & Sons
ISBN 13 : 1118688392
Total Pages : 309 pages
Book Rating : 4.97/5 ( download)

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Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Author : Awtar Krishan
Publisher : John Wiley & Sons
ISBN 13 : 1118148061
Total Pages : 317 pages
Book Rating : 4.68/5 ( download)

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Download or read book Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration written by Awtar Krishan and published by John Wiley & Sons. This book was released on 2011-06-09 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.