Voltage And Patch Clamping With Microelectrodes

Author : Thomas G Smith
Publisher : Springer
ISBN 13 : 1461476011
Total Pages : 263 pages
Book Rating : 4.16/5 ( download)

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Download or read book Voltage and Patch Clamping with Microelectrodes written by Thomas G Smith and published by Springer. This book was released on 2013-05-27 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited and authored by international experts on voltage and patch clamping, this volume is designed to help anyone undertaking experiments requiring the use of these techniques. The only book of its kind to bring together this wealth of information on theory as well as practical techniques, this is a volume that no one involved in voltage and patch clamping can afford to be without.

Author : Areles Molleman
Publisher : John Wiley & Sons
ISBN 13 : 0470856513
Total Pages : 186 pages
Book Rating : 4.12/5 ( download)

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Download or read book Patch Clamping written by Areles Molleman and published by John Wiley & Sons. This book was released on 2003-06-13 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns

Author : Wolfgang Walz
Publisher : Springer Science & Business Media
ISBN 13 : 1592592767
Total Pages : 349 pages
Book Rating : 4.60/5 ( download)

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Download or read book Patch-Clamp Analysis written by Wolfgang Walz and published by Springer Science & Business Media. This book was released on 2008-02-06 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Author : David Ogden
Publisher :
ISBN 13 :
Total Pages : 474 pages
Book Rating : 4.23/5 ( download)

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Download or read book Microelectrode Techniques written by David Ogden and published by . This book was released on 1994 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Yasunobu Okada
Publisher : Springer
ISBN 13 : 9784431539926
Total Pages : 0 pages
Book Rating : 4.21/5 ( download)

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Download or read book Patch Clamp Techniques written by Yasunobu Okada and published by Springer. This book was released on 2012-04-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.

Author : Bert Sakmann
Publisher : Springer Science & Business Media
ISBN 13 : 1461578582
Total Pages : 506 pages
Book Rating : 4.81/5 ( download)

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Download or read book Single-Channel Recording written by Bert Sakmann and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.

Author : Hossein Ardehali
Publisher : John Wiley & Sons
ISBN 13 : 1118495136
Total Pages : 1 pages
Book Rating : 4.31/5 ( download)

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Download or read book Manual of Research Techniques in Cardiovascular Medicine written by Hossein Ardehali and published by John Wiley & Sons. This book was released on 2013-12-16 with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt: While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical “how-to-do-it” review of best-practice techniques for cardiovascular research.

Author : Stefan Dhein
Publisher : Springer
ISBN 13 : 3540265740
Total Pages : 1010 pages
Book Rating : 4.40/5 ( download)

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Download or read book Practical Methods in Cardiovascular Research written by Stefan Dhein and published by Springer. This book was released on 2006-02-28 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.

Author : Mark Dallas
Publisher : Humana
ISBN 13 : 9781071608203
Total Pages : 333 pages
Book Rating : 4.07/5 ( download)

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Download or read book Patch Clamp Electrophysiology written by Mark Dallas and published by Humana. This book was released on 2021-11-13 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.

Author : Toshio Narahashi
Publisher :
ISBN 13 :
Total Pages : 426 pages
Book Rating : 4.98/5 ( download)

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Download or read book Ion Channels of Excitable Cells written by Toshio Narahashi and published by . This book was released on 1994 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because of the highly significant and widely recognized roles of ion channels in physiology, pathophysiology, pharmacology, and toxicology, the term ion channel has now become a household word in the biomedical sciences. This volume covers preparations and techniques for the study of various ion channels. Both voltage-gated and ligand-gated ion channels of neurons, axons, and cardiac and smooth muscles are covered. It includes not only patch clamp techniques but molecular biology and imaging techniques as well. Key Features * Comprehensive protocols included for the study of: * Ion channels using patch-clamp, molecular biology, and imaging techniques * Role of ion channels in physiology, pathophysiology, pharmacology, and toxicology * Specific ion channels of specific tissues