Visualizing Rna Dynamics In The Cell

Author :
Publisher : Academic Press
ISBN 13 : 0128024887
Total Pages : 384 pages
Book Rating : 4.81/5 ( download)

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Download or read book Visualizing RNA Dynamics in the Cell written by and published by Academic Press. This book was released on 2016-05-27 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in visualizing RNA dynamics in the cell Contains sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2 and visualization of 5S dynamics in live cells using photostable corn probe

Author : Jeffrey E. Gerst
Publisher : Humana Press
ISBN 13 : 9781617790065
Total Pages : 498 pages
Book Rating : 4.60/5 ( download)

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Download or read book RNA Detection and Visualization written by Jeffrey E. Gerst and published by Humana Press. This book was released on 2011-08-24 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.

Author : Kiyoshi Nagai
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 302 pages
Book Rating : 4.48/5 ( download)

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Download or read book RNA-protein Interactions written by Kiyoshi Nagai and published by Oxford University Press, USA. This book was released on 1994 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.

Author : Imre Gaspar
Publisher : Humana Press
ISBN 13 : 9781493972128
Total Pages : 492 pages
Book Rating : 4.2X/5 ( download)

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Download or read book RNA Detection written by Imre Gaspar and published by Humana Press. This book was released on 2017-11-15 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Author : Ashty S. Karim
Publisher :
ISBN 13 : 9781071619988
Total Pages : 435 pages
Book Rating : 4.85/5 ( download)

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Download or read book Cell-Free Gene Expression written by Ashty S. Karim and published by . This book was released on 2022 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

Author : Philippe Jeanteur
Publisher : Springer
ISBN 13 : 3540742662
Total Pages : 153 pages
Book Rating : 4.61/5 ( download)

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Download or read book RNA Trafficking and Nuclear Structure Dynamics written by Philippe Jeanteur and published by Springer. This book was released on 2007-11-03 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores nuclear structure and trafficking involving or relevant to RNA and RNPs. Topics include advances and current problems in the structural organization of different subnuclear compartments, Cajal bodies and gems, speckles containing splicing factors, and PML bodies characteristic of ProMyelocytic leukemia. The book also describes the dynamic aspects of RNA trafficking and the latest technologies for live cell imaging of mRNA.

Author : James G. Fujimoto
Publisher : Oxford University Press
ISBN 13 : 0190287780
Total Pages : 440 pages
Book Rating : 4.88/5 ( download)

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Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

Author : Jun Ki Kim
Publisher : Springer Nature
ISBN 13 : 981336064X
Total Pages : 560 pages
Book Rating : 4.48/5 ( download)

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Download or read book Advanced Imaging and Bio Techniques for Convergence Science written by Jun Ki Kim and published by Springer Nature. This book was released on 2021-04-08 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a wide-ranging guide to advanced imaging techniques and related methods with important applications in translational research or convergence science as progress is made toward a new era in integrative healthcare. Conventional and advanced microscopic imaging techniques, including both non-fluorescent (i.e., label-free) and fluorescent methods, have to date provided researchers with specific and quantitative information about molecules, cells, and tissues. Now, however, the different imaging techniques can be correlated with each other and multimodal methods developed to simultaneously obtain diverse and complementary information. In addition, the latest advanced imaging techniques can be integrated with non-imaging techniques such as mass spectroscopic methods, genome editing, organic/inorganic probe synthesis, nanomedicine, and drug discovery. The book will be of high value for researchers in the biological and biomedical sciences or convergence science who need to use these multidisciplinary and integrated techniques or are involved in developing new analytical methods focused on convergence science.

Author :
Publisher : Academic Press
ISBN 13 : 0128211555
Total Pages : 526 pages
Book Rating : 4.57/5 ( download)

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Download or read book Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods written by and published by Academic Press. This book was released on 2020-07-24 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing ß-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways

Author :
Publisher : Academic Press
ISBN 13 : 0128013192
Total Pages : 365 pages
Book Rating : 4.99/5 ( download)

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Download or read book Brain Transcriptome written by and published by Academic Press. This book was released on 2014-08-27 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. This volume, concentrates on the brain transcriptome. Brings together cutting-edge research on the brain transcriptome