The Neuron In Tissue Culture

Author : L. W. Haynes
Publisher :
ISBN 13 :
Total Pages : 780 pages
Book Rating : 4.94/5 ( download)

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Download or read book The Neuron in Tissue Culture written by L. W. Haynes and published by . This book was released on 1999-02-22 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the Handbook Series contains 73 contributions from experts in the range of fields to which neuronal tissue culture may be applied. Twenty-two keynote chapters contain methods, well-illustrated with data from the contributing authors? laboratones, which exemplify ?landmark? applications of the technique. Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum. The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book. The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons. It is essential reading for all those with research interests in neuroscience, including developmental biologists, neurologists, nerve cell physiologists and neuropharmaoologists.

Author : Shohreh Amini
Publisher : Humana
ISBN 13 : 9781071614396
Total Pages : 205 pages
Book Rating : 4.98/5 ( download)

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Download or read book Neuronal Cell Culture written by Shohreh Amini and published by Humana. This book was released on 2022-06-09 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

Author : Gary Banker
Publisher : MIT Press
ISBN 13 : 9780262024389
Total Pages : 706 pages
Book Rating : 4.81/5 ( download)

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Download or read book Culturing Nerve Cells written by Gary Banker and published by MIT Press. This book was released on 1998 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

Author : Gordon Sato
Publisher : Springer Science & Business Media
ISBN 13 : 1468429043
Total Pages : 298 pages
Book Rating : 4.46/5 ( download)

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Download or read book Tissue Culture of the Nervous System written by Gordon Sato and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad vantages of these cultures.

Author : Sergey Fedoroff
Publisher : Springer Science & Business Media
ISBN 13 : 1475725868
Total Pages : 284 pages
Book Rating : 4.65/5 ( download)

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Download or read book Protocols for Neural Cell Culture written by Sergey Fedoroff and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.

Author : Phillip G. Nelson
Publisher : Springer Science & Business Media
ISBN 13 : 1468438034
Total Pages : 431 pages
Book Rating : 4.31/5 ( download)

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Download or read book Excitable Cells in Tissue Culture written by Phillip G. Nelson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tissue culture approach to the study of membrane properties of excitable cells has progressed beyond the technical problems of culture methodology. Recent developments have fostered substantive contributions in research con cerned with the physiology, pharmacology, and biophysics of cell membranes in tissue culture. The scope of this volume is related to the application of tissue culture methodology to developmental processes and cellular mechanisms of electrical and chemical excitability. The major emphasis will be on the body of new biological information made available by the analytic possibilities inherent in the tissue culture systems. Naturally occurring preparations of excitable cells are frequently of suf ficient morphological complexity to compromise the analysis of the data obtained from them. Some of the limitations associated with dissected prepa rations have to do with the direct visualization of and access to the cell(s) in question and maintenance of steady-state conditions for prolonged periods of time. Since preparations in tissue culture can circumvent these problems, it is feasible to analyze the properties of identifiable cells, grown either singly or in prescribed geometries, as well as to follow the development of cellular inter actions. A crucial consideration in the use of cultured preparations is that they must faithfully capture the phenomenon of interest to the investigator. This and other potential limitations on the methodology are of necessary concern in the present volume.

Author : Jane Bottenstein
Publisher : Springer Science & Business Media
ISBN 13 : 1461324734
Total Pages : 392 pages
Book Rating : 4.37/5 ( download)

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Download or read book Cell Culture in the Neurosciences written by Jane Bottenstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.

Author : William M. Reichert
Publisher : CRC Press
ISBN 13 : 1420009303
Total Pages : 300 pages
Book Rating : 4.09/5 ( download)

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Download or read book Indwelling Neural Implants written by William M. Reichert and published by CRC Press. This book was released on 2007-12-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite enormous advances made in the development of external effector prosthetics over the last quarter century, significant questions remain, especially those concerning signal degradation that occurs with chronically implanted neuroelectrodes. Offering contributions from pioneering researchers in neuroprosthetics and tissue repair, Indwel

Author : Alan L. Harvey
Publisher : Springer Science & Business Media
ISBN 13 : 1468488104
Total Pages : 317 pages
Book Rating : 4.04/5 ( download)

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Download or read book The Pharmacology of Nerve and Muscle in Tissue Culture written by Alan L. Harvey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of tissue culture were introduced at the beginning of this century. They have become more and more popular as it is realized that they are not as difficult or as esoteric as some early protagonists liked to maintain. Most of the work performed with culture methods has simply concerned cell growth and survival. Biologists have long used culture approaches to provide a simple system in which to study cell division and multiplication. Any pharmacology done on cultured tissue was largely toxicological or as part of a screening programme for poten tial anti-cancer drugs. In the last decade there has been a great increase in the use of excitable cells in tissue culture. Nerves and muscles from a wide variety of sources can maintain their highly differentiated properties in culture. Such cultures offer an attractive preparation for use in physiological and pharmacological investigations. Consequently, a vast amount of work has been produced, and this book is an attempt to review it. It is hoped that this will introduce physiologists and pharmacologists to the potential of culture methods for their experiments and also indicate to more traditional tissue culture users further possible areas of interest. By being more comprehensive in scope and by trying to concentrate largely on drug actions, I hope that the present volume usefully extends the treatment of the subject begun earlier in the excellent works by Crain (1976) and Nelson and Lieberman (1981).

Author : Michael Aschner
Publisher : Humana
ISBN 13 : 9781493992300
Total Pages : 302 pages
Book Rating : 4.09/5 ( download)

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Download or read book Cell Culture Techniques written by Michael Aschner and published by Humana. This book was released on 2020-05-10 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.