Single Molecule Studies Of Nucleic Acids And Their Proteins

Author : David Bensimon
Publisher : Oxford University Press
ISBN 13 : 0192559966
Total Pages : 224 pages
Book Rating : 4.68/5 ( download)

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Download or read book Single-Molecule Studies of Nucleic Acids and Their Proteins written by David Bensimon and published by Oxford University Press. This book was released on 2018-11-30 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Author : BENSIMON ET AL.
Publisher :
ISBN 13 : 9780191864711
Total Pages : pages
Book Rating : 4.14/5 ( download)

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Download or read book SINGLE MOLECULE STUDIES OF NUCLEIC ACIDS AND THEIR PROTEINS. written by BENSIMON ET AL. and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : David Bensimon
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.13/5 ( download)

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Download or read book Single Molecule Studies of Nucleic Acids and Their Associated Proteins written by David Bensimon and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive overview of the foundations of single-molecule studies, based on manipulation of the molecules and observation of these with fluorescent probes. It first discusses the forces present at the single-molecule scale, the methods to manipulate them, and their pros and cons. It goes on to present an introduction to single-molecule fluorescent studies based on a quantum description of absorption and emission of radiation due to Einstein. Various considerations in the study of single molecules are introduced (including signal to noise, non-radiative decay, triplet states, etc.) and some novel super-resolution methods are sketched. The elastic and dynamic properties of polymers, their relation to experiments on DNA and RNA, and the structural transitions observed in those molecules upon stretching, twisting, and unzipping are presented. The use of these single-molecule approaches for the investigation of DNA-protein interactions is highlighted via the study of DNA and RNA polymerases, helicases, and topoisomerases. Beyond the confirmation of expected mechanisms (e.g., the relaxation of DNA torsion by topoisomerases in quantized steps) and the discovery of unexpected ones (e.g., strand-switching by helicases, DNA scrunching by RNA polymerases, and chiral discrimination by bacterial topoII), these approaches have also fostered novel (third generation) sequencing technologies.

Author : Iddo Heller
Publisher : Springer Nature
ISBN 13 : 1071633775
Total Pages : 511 pages
Book Rating : 4.79/5 ( download)

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Download or read book Single Molecule Analysis written by Iddo Heller and published by Springer Nature. This book was released on 2023-11-13 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Alexander E. Knight
Publisher : Academic Press
ISBN 13 : 008092123X
Total Pages : 369 pages
Book Rating : 4.35/5 ( download)

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Download or read book Single Molecule Biology written by Alexander E. Knight and published by Academic Press. This book was released on 2009-02-26 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. Organized by biological system or molecule Each chapter discusses insights into mechanism of action, structure, and function Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc Includes an introduction to key principles and an historical overview Discusses applications in molecular diagnostics and drug discovery Provides an expert's perspective on future development

Author : Erwin J. G. Peterman
Publisher : Humana Press
ISBN 13 : 9781617792816
Total Pages : 317 pages
Book Rating : 4.10/5 ( download)

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Download or read book Single Molecule Analysis written by Erwin J. G. Peterman and published by Humana Press. This book was released on 2011-09-15 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Author : Daniel Cibrán Pérez González
Publisher :
ISBN 13 :
Total Pages : 194 pages
Book Rating : 4.29/5 ( download)

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Download or read book Single-molecule Studies of Nucleic Acid Folding and Nucleic Acid-protein Interactions written by Daniel Cibrán Pérez González and published by . This book was released on 2017 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Toshio Yanagida
Publisher : John Wiley & Sons
ISBN 13 : 3527312889
Total Pages : 347 pages
Book Rating : 4.87/5 ( download)

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Download or read book Single Molecule Dynamics in Life Science written by Toshio Yanagida and published by John Wiley & Sons. This book was released on 2008-11-24 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Author : Wolfram Saenger
Publisher :
ISBN 13 :
Total Pages : 230 pages
Book Rating : 4.28/5 ( download)

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Download or read book Protein-nucleic Acid Interaction written by Wolfram Saenger and published by . This book was released on 1989 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a series of essays which describe a range of problems in the field of nucleic-acid interactions, investigated by a variety of techniques. An introductory chapter on DNA-protein interactions in the regulation of gene expression is followed by papers on selected model systems.

Author : Peter Caton Anthony
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.54/5 ( download)

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Download or read book Single-molecule Studies of Nucleic Acid Folding written by Peter Caton Anthony and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acids--DNA and RNA--are critical to life, involved in the storage and decoding of genetic information in the cell as well as the regulation and catalysis of specific biological processes. The function of a nucleic acid molecule is determined in large part by the structure it adopts, which in turn depends on its sequence. The mechanisms of sequence-directed nucleic acid folding remain incompletely understood, particularly for large RNA molecules. The work presented in this thesis uses single-molecule optical-trapping techniques to study nucleic acid folding, where reversible folding is induced and measured in individual molecules through the application of force. In Chapter 2 we demonstrate direct measurement of the full folding energy landscapes of DNA hairpins, which comprise a model system for studying nucleic acid secondary structure, and show how such landscapes are sensitive to sequence. In Chapter 3 we study the electrostatics of DNA hairpin folding by measuring trends in folding energies under different ionic conditions and comparing these trends with those predicted by Poisson-Boltzmann theory. Finally, in Chapter 4 we examine folding of the TPP riboswitch aptamer, an RNA molecule with complex secondary structure that also adopts tertiary structure upon binding a small-molecule ligand. We measured the folding energy landscape of the aptamer and perturbations of this landscape resulting from mutations and ligand binding, and propose a kinetic model to describe the coupling between aptamer folding and ligand binding. Taken together, the results presented here demonstrate the usefulness of the energy landscape framework for characterizing nucleic acid folding in conjunction with single-molecule measurements.