Rna Interference Editing And Modification

Author : Jonatha M. Gott
Publisher : Springer Science & Business Media
ISBN 13 : 1592597750
Total Pages : 437 pages
Book Rating : 4.58/5 ( download)

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Download or read book RNA Interference, Editing, and Modification written by Jonatha M. Gott and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a comprehensive collection of cuttingedge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification. Readily reproducible protocols for discovering new genes or altering gene expression.

Author : Kamel A Abd-Elsalam
Publisher : Elsevier
ISBN 13 : 0128219114
Total Pages : 842 pages
Book Rating : 4.19/5 ( download)

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Download or read book CRISPR and RNAi Systems written by Kamel A Abd-Elsalam and published by Elsevier. This book was released on 2021-02-27 with total page 842 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

Author : Aditi Singh
Publisher : BoD – Books on Demand
ISBN 13 : 1838806962
Total Pages : 136 pages
Book Rating : 4.65/5 ( download)

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Download or read book Modulating Gene Expression written by Aditi Singh and published by BoD – Books on Demand. This book was released on 2019-05-29 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

Author : Mohammad W. Khan
Publisher :
ISBN 13 : 9781838806989
Total Pages : 134 pages
Book Rating : 4.89/5 ( download)

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Download or read book Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies written by Mohammad W. Khan and published by . This book was released on 2019 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

Author : Scott Ryan Suter
Publisher :
ISBN 13 : 9780438637276
Total Pages : pages
Book Rating : 4.75/5 ( download)

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Download or read book The Design and Study of Chemically Modified RNA for RNA Interference and RNA Editing Using Structure Guided Approaches written by Scott Ryan Suter and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acids have been studied extensively due to their numerous functional roles in biological systems. Biological processes associated with the post transcriptional modification of RNA, namely RNA interference (RNAi) and RNA editing, have shown therapeutic potential due to their ability to alter mRNAs. RNA interference is a process which results in the cleavage of mRNA transcripts using a guide RNA template to find its target sequence and prevent translational expression. RNA editing causes the recoding of mRNA transcripts, altering protein function. The following dissertation discusses experiments which guide the chemical modification of RNA substrates of RNAi and RNA editing to bypass hurdles associated with their development as therapeutics. The Beal lab studies nucleobase modifications of short interfering RNAs (siRNAs), which are used in RNAi to target mRNA sequences of interest. Chapters 2 and 3 of this thesis will discuss nucleobase modifications to bypass the innate immune response of the cell and prevent off-target knockdown of undesired mRNA sequences. Using the crystal structures of an innate immune signaling protein, Toll-like Receptor 8 (TLR8), and of the catalytic endonuclease of RNAi, Argonaute 2 (Ago2), new chemical modifications for siRNAs were synthesized which can bypass TLR8 recognition and allow selective target mRNA cleavage by Ago2. Crystal structures of TLR8 bound to a small molecule and RNA derived agonists allowed the rational design of major and minor groove nucleobase modifications for siRNAs to bypass TLR8 recognition. To study the off-target knockdown of mRNA transcripts by Ago2, ethynyl modified riboses and nucleobases within key positions of the siRNA sequence were synthesized. Crystal structures of guide RNA loaded hAgo2 bound to an off-target mimic were vital for the study of these new triazoles. This resulted in the development of new siRNAs which were able to select for the knockdown of their targeted sequence over known off-target sequences. Chapter 4 of this dissertation discusses preliminary work in the use of small molecule molecular docking software to find nucleobase modifications to enhance RNA/protein interactions. New RNAs which can bind to guide strand loaded Ago2 were synthesized using this screening method and were found to bind more tightly than three of the four canonical RNA bases. This screening method was also applied to the RNA editing protein adenosine deaminase acting on RNA 2 (ADAR2). The recently solved crystal structure of ADAR2’s catalytic domain bound to its RNA substrate displayed a potential binding pocket to which modified, non-edited RNAs could bind. This work discusses the preliminary results of these modified non-edited RNAs, and how they influence the deamination reaction of ADAR2. This chapter also discusses the use of the transition state inhibitor 8-aza-nebularine from the solved crystal structure, as a ligand to accomplish a molecular docking screen to search for potential inhibitors of ADAR2.

Author : Mouldy Sioud
Publisher : Humana
ISBN 13 : 9781071602928
Total Pages : 486 pages
Book Rating : 4.26/5 ( download)

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Download or read book RNA Interference and CRISPR Technologies written by Mouldy Sioud and published by Humana. This book was released on 2021-02-15 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine.

Author : Jonatha M. Gott
Publisher : Humana
ISBN 13 : 9781588292421
Total Pages : 436 pages
Book Rating : 4.28/5 ( download)

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Download or read book RNA Interference, Editing, and Modification written by Jonatha M. Gott and published by Humana. This book was released on 2004-04-15 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of cutting-edge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification.

Author : Yanqiang Li
Publisher : Frontiers Media SA
ISBN 13 : 2832505430
Total Pages : 340 pages
Book Rating : 4.34/5 ( download)

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Download or read book RNA Editing and Modification in Development and Diseases written by Yanqiang Li and published by Frontiers Media SA. This book was released on 2022-11-14 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author :
Publisher : Academic Press
ISBN 13 : 0128023317
Total Pages : 441 pages
Book Rating : 4.10/5 ( download)

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Download or read book RNA Modification written by and published by Academic Press. This book was released on 2015-12-11 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Modification provides a useful examination of the science and its role in biological regulation, the current frontier of life science research, and includes various RNA modications and their role in gene expression. It represents the most up-to-date knowledge and protocols available today. Dynamic RNA modifications and their roles in biological regulation are the current frontier of life science research This volume of Methods in Enzymology represents up to date knowledge and protocols

Author :
Publisher : Academic Press
ISBN 13 : 0128235861
Total Pages : 476 pages
Book Rating : 4.67/5 ( download)

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Download or read book RNA Modification Enzymes written by and published by Academic Press. This book was released on 2021-09-11 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Modification Enzymes, Volume 659 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics, including Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis, Development of RNA modification mapping pipelines for high-throughput sequencing approaches, AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications, Facile detection of RNA phospho-methylation in cells, Detection and analysis of glycosylated queuosine modifications, A comprehensive pipeline for analysis of RNA 3’-end modification, Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on the RNA Modification Enzymes