Rat Hybridomas And Rat Monoclonal Antibodies 1990

Author : Herve Bazin
Publisher : CRC Press
ISBN 13 : 1351358286
Total Pages : 786 pages
Book Rating : 4.86/5 ( download)

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Download or read book Rat Hybridomas and Rat Monoclonal Antibodies (1990) written by Herve Bazin and published by CRC Press. This book was released on 2017-11-22 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.

Author : Taylor & Francis Group
Publisher :
ISBN 13 : 9781138561649
Total Pages : pages
Book Rating : 4.49/5 ( download)

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Download or read book Revival written by Taylor & Francis Group and published by . This book was released on 2018-12-31 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Herve Bazin
Publisher : CRC Press
ISBN 13 : 9781138506107
Total Pages : 534 pages
Book Rating : 4.09/5 ( download)

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Download or read book Rat Hybridomas and Rat Monoclonal Antibodies (1990) written by Herve Bazin and published by CRC Press. This book was released on 2017-09-20 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.

Author : A.M. Campbell
Publisher : Elsevier
ISBN 13 : 9780080858821
Total Pages : 264 pages
Book Rating : 4.21/5 ( download)

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Download or read book Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas written by A.M. Campbell and published by Elsevier. This book was released on 2000-04-01 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Author : Herve Bazin
Publisher : CRC Press
ISBN 13 : 1351358294
Total Pages : 534 pages
Book Rating : 4.93/5 ( download)

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Download or read book Rat Hybridomas and Rat Monoclonal Antibodies (1990) written by Herve Bazin and published by CRC Press. This book was released on 2017-11-22 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309173051
Total Pages : 74 pages
Book Rating : 4.56/5 ( download)

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Download or read book Monoclonal Antibody Production written by National Research Council and published by National Academies Press. This book was released on 1999-05-06 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.

Author : Ailsa M. Campbell
Publisher :
ISBN 13 : 9780444805928
Total Pages : 265 pages
Book Rating : 4.23/5 ( download)

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Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by Ailsa M. Campbell and published by . This book was released on 1969 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Author : James W. Goding
Publisher : Elsevier
ISBN 13 : 0080536956
Total Pages : 513 pages
Book Rating : 4.58/5 ( download)

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Download or read book Monoclonal Antibodies written by James W. Goding and published by Elsevier. This book was released on 1996-02-26 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology. The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production and application of monoclonal antibodies. As in previous editions, detailed and critical accounts of the theory, production, purification, fragmentation, storage and radiolabelling of monoclonal antibodies are given, along with descriptions of their use in antigen characterization, affinity chromatography and immunofluorescence. The present volume has been comprehensively updated to cover recent rapid advances, particularly with respect to the applications of molecular biology, the use of antibodies in closing and heterologous expression of genes, immunohistology and phage display libraries. Since the previous edition, there has been a growing trend towards the replacement of procedures using radioactive isotopes, and the current edition incorporates these newer technologies. The text is oriented towards problems solving, and makes it easy to adapt each procedure to individual needs. Extensive cross-referencing, a glossary and a comprehensive index make this book an essential reference. This book will be vital both for laboratories already producing or using monoclonal antibodies, and for workers in many disciplines who are contemplating their use. Provides an integrated treatment of both the production and application of monoclonals in cell biology, biochemistry, and immunology Gives detailed and critical accounts of the theory, production, purification, storage, and relabelling of monoclonals, and their use in antigen characterization, affinity chromorography, and immunofluroscence Comprehensively updated to cover the rapid advances that have occurred since the publication of the Second Edition

Author : Johann H. Peters
Publisher : Springer Science & Business Media
ISBN 13 : 3642745326
Total Pages : 504 pages
Book Rating : 4.24/5 ( download)

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Download or read book Monoclonal Antibodies written by Johann H. Peters and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present new version of this popular laboratory manual is at the same time the first one of this text in the English language - and this makes me even a little proud, as it reminds me of probably the first collection of monoclonal recipes in English, written by myself, which circulated for a couple of years in many laboratories. In the meantime many researchers have put enormous effort into improving methods for monoclonal antibody production. The proce dures have become more and more standardized and by this have more and more lost the character of magic secrets. Hinrich Peters and Horst Baumgarten, who had followed this good tradition already in the previous edition, written in German, suc ceeded in making laboratory tricks teachable. They had contributed their own experiences in cell culture and immunology, and were able to engage a number of experienced authors to contribute to the work. They were all willing to follow the general concept of this book, which contains a brief theoretical background for the methods described and presents the procedures in a highly organized structure. So the book has retained its shape as a "cook-book", which I especially like.

Author : William R Strohl
Publisher : Elsevier
ISBN 13 : 1908818093
Total Pages : 697 pages
Book Rating : 4.96/5 ( download)

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Download or read book Therapeutic Antibody Engineering written by William R Strohl and published by Elsevier. This book was released on 2012-10-16 with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering. Goes beyond the standard engineering issues covered by most books and delves into structure-function relationships Integration of knowledge across all areas of antibody engineering, development, and marketing Discusses how current and future genetic engineering of cell lines will pave the way for much higher productivity