Protocols For Nucleic Acid Analysis By Nonradioactive Probes

Author : Elena Hilario
Publisher : Springer Science & Business Media
ISBN 13 : 1597452297
Total Pages : 318 pages
Book Rating : 4.98/5 ( download)

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Download or read book Protocols for Nucleic Acid Analysis by Nonradioactive Probes written by Elena Hilario and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Author : Christoph Kessler
Publisher : Springer Science & Business Media
ISBN 13 : 3642572065
Total Pages : 759 pages
Book Rating : 4.67/5 ( download)

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Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.

Author : Robert H. Symons
Publisher : CRC Press
ISBN 13 : 9780849349423
Total Pages : 246 pages
Book Rating : 4.27/5 ( download)

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Download or read book Nucleic Acid Probes written by Robert H. Symons and published by CRC Press. This book was released on 1989-03-31 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: With each chapter written by a distinguished expert in the biochemistry field, this comprehensive volume describes the preparation and use of a variety of radioactive and non-radioactive probes in situations ranging from research laboratories to routine diagnosis laboratories. The enzymatic and chemical techniques for labeling nucleic acid probes with radioisotopes and with non-radioactive ligands and haptens are discussed. Additionally, the associated methods for their detection, the use of these probes in the diagnosis of human and microbial pathogens, of plant viruses and viroids, and of human genetic disorders, as well as in the detection of nucleic acids in tissues and cells by in situ hybridization are presented.

Author : George H. Keller
Publisher :
ISBN 13 :
Total Pages : 696 pages
Book Rating : 4.85/5 ( download)

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Download or read book DNA Probes written by George H. Keller and published by . This book was released on 1993 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition is an updated version of the first edition, plus many new sections on specific applications of nucleic acid probes. Due to the explosion of such applications, experts in each major area have described their specialty. As in the first edition, the emphasis is on the commercial uses of DNA probes, as in diagnostic applications, and it incorporates background material, advice and specific protocols.

Author : Larry J. Kricka
Publisher : Academic Press
ISBN 13 : 0323137717
Total Pages : 373 pages
Book Rating : 4.13/5 ( download)

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Download or read book Nonisotopic DNA Probe Techniques written by Larry J. Kricka and published by Academic Press. This book was released on 2012-12-02 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided. This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection. Gives extensive practical information Covers major types of nonisotopic labels and procedures Presents background information for each method Provides strategies and detailed experimental procedures for labeling and detecting DNA sequences by Fluorescence Chemiluminescence Bioluminescence Colorimetry

Author : Christoph Kessler
Publisher : Springer
ISBN 13 : 3662001446
Total Pages : 449 pages
Book Rating : 4.48/5 ( download)

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Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 2012-12-06 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.

Author : P. Tijssen
Publisher : Elsevier
ISBN 13 : 9780080858937
Total Pages : 344 pages
Book Rating : 4.37/5 ( download)

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Download or read book Hybridization with Nucleic Acid Probes, Part II written by P. Tijssen and published by Elsevier. This book was released on 1993-10-21 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools. These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.

Author : Ralph Rapley
Publisher : Springer
ISBN 13 : 1592596428
Total Pages : 708 pages
Book Rating : 4.23/5 ( download)

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Download or read book Molecular Biomethods Handbook written by Ralph Rapley and published by Springer. This book was released on 2007-10-09 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.85/5 ( download)

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Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Author : Dmitry M. Kolpashchikov
Publisher : Humana
ISBN 13 : 9781493960415
Total Pages : 0 pages
Book Rating : 4.15/5 ( download)

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Download or read book Nucleic Acid Detection written by Dmitry M. Kolpashchikov and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.