Nucleic Acid Hybridization

Author : M.L.M. Anderson
Publisher : Garland Science
ISBN 13 : 1000144550
Total Pages : 279 pages
Book Rating : 4.50/5 ( download)

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Download or read book Nucleic Acid Hybridization written by M.L.M. Anderson and published by Garland Science. This book was released on 2020-08-26 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1998. This book is targeted at the beginner who has little or no knowledge of the principles or practice of hybridization. This text concentrates on solution and filter hybridization with a final chapter on current developments which includes DNA chips and advances in probe design.

Author : Anton Buzdin
Publisher : Springer Science & Business Media
ISBN 13 : 1402060408
Total Pages : 328 pages
Book Rating : 4.03/5 ( download)

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Download or read book Nucleic Acids Hybridization written by Anton Buzdin and published by Springer Science & Business Media. This book was released on 2007-06-07 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.

Author : H. Ursprung
Publisher : Springer Science & Business Media
ISBN 13 : 3540371494
Total Pages : 83 pages
Book Rating : 4.96/5 ( download)

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Download or read book Nucleic Acid Hybridization in the Study of Cell Differentiation written by H. Ursprung and published by Springer Science & Business Media. This book was released on 2013-06-05 with total page 83 pages. Available in PDF, EPUB and Kindle. Book excerpt: The informational content of cells is encoded in the nucleotide sequences of their DNA. The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription. In the test tube, these restrictions can be exploited for ascertaining similarities and dissimilarities of nucleic acids of varying origin by measuring the kinetics of reassociation of polynucleotides to double-stranded molecules in DNA DNA renaturation or RNA-DNA hybridization experiments, and by determining the thermal stability and other physical-chemical properties of the resulting hybrid molecules. This method has enormous potential for developmental biology. It offers a more direct approach to the ever-present question of the genetic identity of different cell types in an individual organism, and a more direct test of the hypothesis of diffe rential gene function. It offers the possibility of localizing genes on chromosomes without the use of Mendelian genetics. It is an indispensable tool in the isolation, purification, and characterization of genes. This volume brings together six articles by investigators actively working on various aspects of developmental biology who use nucleic acid hybridization as a tool in their research. Sound in theory, the method is in a honing phase as regards the technical detail. This is expressed in the hesitation with which some of the conclusions are rightly drawn.

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.85/5 ( download)

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Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Author : Christoph Kessler
Publisher : Springer Science & Business Media
ISBN 13 : 9783540646013
Total Pages : 1170 pages
Book Rating : 4.19/5 ( download)

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Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2000-03-11 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.

Author : P. Tijssen
Publisher : Elsevier
ISBN 13 : 9780080858937
Total Pages : 344 pages
Book Rating : 4.37/5 ( download)

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Download or read book Hybridization with Nucleic Acid Probes, Part II written by P. Tijssen and published by Elsevier. This book was released on 1993-10-21 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools. These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.

Author : P. M. Gilmartin
Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 166 pages
Book Rating : 4.66/5 ( download)

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Download or read book Nucleic Acid Hybridization written by P. M. Gilmartin and published by Wiley-Interscience. This book was released on 1996-03-19 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: This quick reference guide covers the fundamental techniques and practical tips needed to undertake a variety of nucleic acid hybridization methods.

Author : J. Ross
Publisher :
ISBN 13 :
Total Pages : 188 pages
Book Rating : 4.87/5 ( download)

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Download or read book Nucleic Acid Hybridization written by J. Ross and published by . This book was released on 1998-03-11 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handy laboratory manual allows quick and easy access to the most important techniques in molecular hybridization. It briefly describes each technique, explaining the advantages and disadvantages and avoiding unnecessary detail. It also explains why certain techniques work (or fail to work).

Author : Heinrich Ursprung
Publisher :
ISBN 13 : 9780387057422
Total Pages : 76 pages
Book Rating : 4.20/5 ( download)

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Download or read book Nucleic Acid Hybridization in the Study of Cell Differentiation written by Heinrich Ursprung and published by . This book was released on 1972 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Chung Eun Ha
Publisher : Academic Press
ISBN 13 : 0080916880
Total Pages : 600 pages
Book Rating : 4.80/5 ( download)

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Download or read book Essentials of Medical Biochemistry written by Chung Eun Ha and published by Academic Press. This book was released on 2011-01-28 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expert biochemist N.V. Bhagavan’s new work condenses his successful Medical Biochemistry texts along with numerous case studies, to act as an extensive review and reference guide for both students and experts alike. The research-driven content includes four-color illustrations throughout to develop an understanding of the events and processes that are occurring at both the molecular and macrolecular levels of physiologic regulation, clinical effects, and interactions. Using thorough introductions, end of chapter reviews, fact-filled tables, and related multiple-choice questions, Bhagavan provides the reader with the most condensed yet detailed biochemistry overview available. More than a quick survey, this comprehensive text includes USMLE sample exams from Bhagavan himself, a previous coauthor. Clinical focus emphasizing relevant physiologic and pathophysiologic biochemical concepts Interactive multiple-choice questions to prep for USMLE exams Clinical case studies for understanding basic science, diagnosis, and treatment of human diseases Instructional overview figures, flowcharts, and tables to enhance understanding