Nuclear Reprogramming

Author : Kejin Hu
Publisher :
ISBN 13 : 9781071610848
Total Pages : 306 pages
Book Rating : 4.48/5 ( download)

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Download or read book Nuclear Reprogramming written by Kejin Hu and published by . This book was released on 2021 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field. .

Author : Paul J. Verma
Publisher : Springer Science & Business Media
ISBN 13 : 1597451541
Total Pages : 684 pages
Book Rating : 4.43/5 ( download)

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Download or read book Nuclear Transfer Protocols written by Paul J. Verma and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

Author : Justin Ainscough
Publisher : Springer Science & Business Media
ISBN 13 : 161779225X
Total Pages : 340 pages
Book Rating : 4.50/5 ( download)

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Download or read book Nuclear Reprogramming and Stem Cells written by Justin Ainscough and published by Springer Science & Business Media. This book was released on 2011-09-02 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved. Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.

Author : Steve Pells
Publisher : Humana
ISBN 13 : 9781588293794
Total Pages : 334 pages
Book Rating : 4.93/5 ( download)

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Download or read book Nuclear Reprogramming written by Steve Pells and published by Humana. This book was released on 2005-12-05 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.

Author : Gregory R. Bock
Publisher : John Wiley & Sons
ISBN 13 : 0470091444
Total Pages : 230 pages
Book Rating : 4.49/5 ( download)

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Download or read book Stem Cells written by Gregory R. Bock and published by John Wiley & Sons. This book was released on 2005-09-01 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding stem cells at the molecular level is essential to understanding their behaviour in a physiological context. This volume in our acclaimed Novartis Foundation series features animated discussion from the world’s experts in this topic on the important ethical issues that are raised by research on stem cells. They review the various regulatory regimes, which apply in different countries – a key factor in determining where future stem cell research is carried out. Potential clinical applications covered in the book include the production of cardiomyocytes to replace damaged heart tissue, the production of insulin-producing cells for patients with diabetes, and the generation of neurons for the treatment of patients with Parkinson’s disease or spinal cord injury. Particular attention is paid to the factors that maintain stem cells in a pluripotent state or which drive them to create differentiated and lineage-committed cells in vitro and in vivo. Nuclear reprogramming, the process by which a nucleus acquires developmental potential, is covered here as well. It is relevant to stem cell research generally, and also to research on the cloning of animals by nuclear transfer. This book is an essential purchase for all those engaged in stem cell research, whether in the laboratory, the clinic or the regulatory authorities. From the reviews: "...this book provides: a comprehensive overview of current issues in stem cell research, with contributions from leading figures..." —BRITISH SOCIETY OF CELL BIOLOGY

Author : Steve Pells
Publisher : Springer Science & Business Media
ISBN 13 : 1597450057
Total Pages : 337 pages
Book Rating : 4.58/5 ( download)

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Download or read book Nuclear Reprogramming written by Steve Pells and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.

Author : L. Gianaroli
Publisher : Raven Press (ID)
ISBN 13 :
Total Pages : 352 pages
Book Rating : 4.97/5 ( download)

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Download or read book Implantation in Mammals written by L. Gianaroli and published by Raven Press (ID). This book was released on 1993 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Carlos Simón
Publisher : Cambridge University Press
ISBN 13 : 1107034477
Total Pages : 199 pages
Book Rating : 4.71/5 ( download)

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Download or read book Stem Cells in Reproductive Medicine written by Carlos Simón and published by Cambridge University Press. This book was released on 2013-07-04 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell science has the potential to impact human reproductive medicine significantly - cutting edge technologies allow the production and regeneration of viable gametes from human stem cells offering potential to preciously infertile patients. Written by leading experts in the field Stem Cells in Reproductive Medicine brings together chapters on the genetics and epigenetics of both the male and female gametes as well as advice on the production and regeneration of gene cells in men and women, trophoblasts and endometrium from human embryonic and adult stem cells. Although focussing mainly on the practical elements of the use of stem cells in reproductive medicine, the book also contains a section on new developments in stem cell research. The book is essential reading for reproductive medicine clinicians, gynecologists and embryologists who want to keep abreast of practical developments in this rapidly developing field.

Author : Nils R. Ringertz
Publisher : Academic Press
ISBN 13 : 1483270092
Total Pages : 381 pages
Book Rating : 4.98/5 ( download)

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Download or read book Cell Hybrids written by Nils R. Ringertz and published by Academic Press. This book was released on 2014-06-28 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Hybrids summarizes the methodology of cell fusion-the fusion of human, animal, and plant cells of different origins to produce cell hybrids-and surveys the main applications and current findings of the hybridization technique. The book opens with a chapter on the history cell hybridization. This is followed by separate chapters on spontaneous cell fusion, virus-induced cell fusion, the cell fusion mechanism, regulation of DNA synthesis and mitosis in heterokaryons and homokaryons, and regulatory events which occur when two cells with different nuclear activity and/or phenotype are fused with each other. Subsequent chapters deal with methods used in preparing various cell fragments and some of their properties and uses in fusion experiments; isolation of growing hybrid cells; chromosome patterns and phenotypic expression in hybrid cells; cell organelles in hybrid cells; analysis of malignancy by cell hybridization. The final chapters discuss the use of somatic cell hybridization to analyze the interaction between a number of viruses and their host cells; and the use of plant cell hybrids.

Author : Elijah Joshua
Publisher : Createspace Independent Publishing Platform
ISBN 13 : 9781548176792
Total Pages : 50 pages
Book Rating : 4.96/5 ( download)

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Download or read book Nuclear Reprogramming written by Elijah Joshua and published by Createspace Independent Publishing Platform. This book was released on 2017-06-12 with total page 50 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.