Nitric Oxide Protocols

Author : M. A Titheradge
Publisher : Springer Science & Business Media
ISBN 13 : 1592597491
Total Pages : 321 pages
Book Rating : 4.99/5 ( download)

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Download or read book Nitric Oxide Protocols written by M. A Titheradge and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.

Author : Helen O. McCarthy
Publisher : Humana Press
ISBN 13 : 9781617379635
Total Pages : 226 pages
Book Rating : 4.38/5 ( download)

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Download or read book Nitric Oxide written by Helen O. McCarthy and published by Humana Press. This book was released on 2010-12-16 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.

Author :
Publisher : World Health Organization
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.33/5 ( download)

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Download or read book WHO Guidelines for Indoor Air Quality written by and published by World Health Organization. This book was released on 2010 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents WHO guidelines for the protection of public health from risks due to a number of chemicals commonly present in indoor air. The substances considered in this review, i.e. benzene, carbon monoxide, formaldehyde, naphthalene, nitrogen dioxide, polycyclic aromatic hydrocarbons (especially benzo[a]pyrene), radon, trichloroethylene and tetrachloroethylene, have indoor sources, are known in respect of their hazardousness to health and are often found indoors in concentrations of health concern. The guidelines are targeted at public health professionals involved in preventing health risks of environmental exposures, as well as specialists and authorities involved in the design and use of buildings, indoor materials and products. They provide a scientific basis for legally enforceable standards.

Author : Aviv Hassid
Publisher : Springer Science & Business Media
ISBN 13 : 1592598072
Total Pages : 251 pages
Book Rating : 4.76/5 ( download)

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Download or read book Nitric Oxide Protocols written by Aviv Hassid and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.

Author : Nathan Bryan
Publisher :
ISBN 13 : 9780615417134
Total Pages : 170 pages
Book Rating : 4.32/5 ( download)

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Download or read book The Nitric Oxide (No) Solution written by Nathan Bryan and published by . This book was released on 2010-11 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eighty-one million Americans have cardiovascular disease, the arterial damage that leads to the heart attacks and strokes that account for 36 percent of all deaths in the U.S. But few people know that boosting the body's levels of a single molecule of nitric oxide can help prevent, control, and even reverse cardiovascular disease.

Author :
Publisher : DIANE Publishing
ISBN 13 : 1428902805
Total Pages : 57 pages
Book Rating : 4.00/5 ( download)

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Download or read book Nitrogen oxides (NOx) why and how they are controlled written by and published by DIANE Publishing. This book was released on 1999 with total page 57 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Jill Lincoln
Publisher : Cambridge University Press
ISBN 13 : 9780521559775
Total Pages : 60 pages
Book Rating : 4.74/5 ( download)

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Download or read book Nitric Oxide in Health and Disease written by Jill Lincoln and published by Cambridge University Press. This book was released on 1997-08-28 with total page 60 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitric oxide has a tantalizing role in health and disease: while many of its wide-ranging effects are well known, there remains much more to explore and to learn about the interactions of this fascinating molecule in physiological and pathophysiological processes. The volume reviews the myriad effects of nitric oxide as a chemical messenger in the central nervous system, peripheral nervous system, immune system and cardiovascular system. Furthermore, it provides a very practical introduction to the procedures and experimental protocols necessary to work with and study nitric oxide and its synthesizing enzyme, nitric oxide synthase, in the laboratory. In this respect the volume is unique, providing as it does a complete single-volume review of the role of nitric oxide in health and disease, and a very practical introduction to the methods and protocols involved in this intriguing and active area of biomedical research.

Author : Kapuganti Jagadis Gupta
Publisher : Methods in Molecular Biology
ISBN 13 : 9781493980901
Total Pages : 216 pages
Book Rating : 4.04/5 ( download)

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Download or read book Plant Nitric Oxide: Methods and Protocols written by Kapuganti Jagadis Gupta and published by Methods in Molecular Biology. This book was released on 2018-05-05 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.

Author : Weltgesundheitsorganisation
Publisher : World Health Organization
ISBN 13 : 9240034226
Total Pages : 300 pages
Book Rating : 4.28/5 ( download)

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Download or read book WHO global air quality guidelines written by Weltgesundheitsorganisation and published by World Health Organization. This book was released on 2021-09-07 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main objective of these updated global guidelines is to offer health-based air quality guideline levels, expressed as long-term or short-term concentrations for six key air pollutants: PM2.5, PM10, ozone, nitrogen dioxide, sulfur dioxide and carbon monoxide. In addition, the guidelines provide interim targets to guide reduction efforts of these pollutants, as well as good practice statements for the management of certain types of PM (i.e., black carbon/elemental carbon, ultrafine particles, particles originating from sand and duststorms). These guidelines are not legally binding standards; however, they provide WHO Member States with an evidence-informed tool, which they can use to inform legislation and policy. Ultimately, the goal of these guidelines is to help reduce levels of air pollutants in order to decrease the enormous health burden resulting from the exposure to air pollution worldwide.

Author : Louis J. Ignarro
Publisher : Academic Press
ISBN 13 : 9780080525037
Total Pages : 1017 pages
Book Rating : 4.32/5 ( download)

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Download or read book Nitric Oxide written by Louis J. Ignarro and published by Academic Press. This book was released on 2000-09-13 with total page 1017 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitric oxide (NO) is a gas that transmits signals in an organism. Signal transmission by a gas that is produced by one cell and which penetrates through membranes and regulates the function of another cell represents an entirely new principle for signaling in biological systems. NO is a signal molecule of key importance for the cardiovascular system acting as a regulator of blood pressure and as a gatekeeper of blood flow to different organs. NO also exerts a series of other functions, such as acting a signal molecule in the nervous system and as a weapon against infections. NO is present in most living creatures and made by many different types of cells. NO research has led to new treatments for treating heart as well as lung diseases, shock, and impotence. Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis. This book is the first comprehensive text on nitric oxide to cover all aspects--basic biology, chemistry, pathobiology, effects on various disease states, and therapeutic implications. Edited by Nobel Laureate Louis J. Ignarro, editor of the Academic Press journal, Nitric Oxide Authored by world experts on nitric oxide Includes an overview of basic principles of biology and chemical biology Covers principles of pathobiology, including the nervous system, cardiovascular function, pulmonary function, and immune defense