Neuronal Cell Culture

Author : Shohreh Amini
Publisher : Humana
ISBN 13 : 9781071614396
Total Pages : 205 pages
Book Rating : 4.98/5 ( download)

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Download or read book Neuronal Cell Culture written by Shohreh Amini and published by Humana. This book was released on 2022-06-09 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

Author : Jane Bottenstein
Publisher : Springer Science & Business Media
ISBN 13 : 1461324734
Total Pages : 392 pages
Book Rating : 4.37/5 ( download)

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Download or read book Cell Culture in the Neurosciences written by Jane Bottenstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.

Author : Gary Banker
Publisher : MIT Press
ISBN 13 : 9780262024389
Total Pages : 706 pages
Book Rating : 4.81/5 ( download)

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Download or read book Culturing Nerve Cells written by Gary Banker and published by MIT Press. This book was released on 1998 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

Author : Shohreh Amini
Publisher :
ISBN 13 : 9781627036405
Total Pages : 165 pages
Book Rating : 4.07/5 ( download)

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Download or read book Neuronal Cell Culture written by Shohreh Amini and published by . This book was released on 2013 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

Author : Sergey Fedoroff
Publisher : Springer Science & Business Media
ISBN 13 : 1592592074
Total Pages : 366 pages
Book Rating : 4.74/5 ( download)

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Download or read book Protocols for Neural Cell Culture written by Sergey Fedoroff and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.

Author : Peter Riederer
Publisher : Springer Science & Business Media
ISBN 13 : 3211452958
Total Pages : 484 pages
Book Rating : 4.50/5 ( download)

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Download or read book Parkinson's Disease and Related Disorders written by Peter Riederer and published by Springer Science & Business Media. This book was released on 2006-11-23 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a comprehensive overview on current clinical and basic research issues related to Parkinson’s disease and its related disorders. The book is the result of the 16th International Congress of Parkinson’s Disease and Related disorders congress 2005. Its contents are suitable for neurologists, psychiatrists, neurosurgeons, basic researchers, geneticians and patients as well as their relatives.

Author : L. W. Haynes
Publisher :
ISBN 13 :
Total Pages : 780 pages
Book Rating : 4.94/5 ( download)

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Download or read book The Neuron in Tissue Culture written by L. W. Haynes and published by . This book was released on 1999-02-22 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the Handbook Series contains 73 contributions from experts in the range of fields to which neuronal tissue culture may be applied. Twenty-two keynote chapters contain methods, well-illustrated with data from the contributing authors? laboratones, which exemplify ?landmark? applications of the technique. Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum. The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book. The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons. It is essential reading for all those with research interests in neuroscience, including developmental biologists, neurologists, nerve cell physiologists and neuropharmaoologists.

Author : William M. Reichert
Publisher : CRC Press
ISBN 13 : 1420009303
Total Pages : 300 pages
Book Rating : 4.09/5 ( download)

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Download or read book Indwelling Neural Implants written by William M. Reichert and published by CRC Press. This book was released on 2007-12-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite enormous advances made in the development of external effector prosthetics over the last quarter century, significant questions remain, especially those concerning signal degradation that occurs with chronically implanted neuroelectrodes. Offering contributions from pioneering researchers in neuroprosthetics and tissue repair, Indwel

Author : Manfred Gerlach
Publisher : Springer Science & Business Media
ISBN 13 : 3211735747
Total Pages : 326 pages
Book Rating : 4.49/5 ( download)

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Download or read book Neuropsychiatric Disorders written by Manfred Gerlach and published by Springer Science & Business Media. This book was released on 2007-12-28 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This multidisciplinary book includes current research papers and reviews in the areas of basic neuroscience, neural mechanisms underlying neurodegenerative disorders. It further includes new approaches for neuroprotective treatments, clinical, neurobiological and treatment aspects of psychiatric disorders. The book was conceived as a celebration of the professional life and work of Peter Riederer to mark the occasion of his retirement.

Author : Irwin B. Levitan
Publisher : Oxford University Press, USA
ISBN 13 : 9780195145236
Total Pages : 640 pages
Book Rating : 4.32/5 ( download)

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Download or read book The Neuron written by Irwin B. Levitan and published by Oxford University Press, USA. This book was released on 2002 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intended for use by advanced undergraduate, graduate and medical students, this book presents a study of the unique biochemical and physiological properties of neurons, emphasising the molecular mechanisms that generate and regulate their activity.