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Author : Gary C. Howard
Publisher : McGraw-Hill/Appleton & Lange
ISBN 13 : 9780838569467
Total Pages : 342 pages
Book Rating : 4.63/5 ( download)
Download or read book Methods in Nonradioactive Detection written by Gary C. Howard and published by McGraw-Hill/Appleton & Lange. This book was released on 1993-01-01 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Elena Hilario
Publisher : Springer Science & Business Media
ISBN 13 : 1597452297
Total Pages : 318 pages
Book Rating : 4.98/5 ( download)
Download or read book Protocols for Nucleic Acid Analysis by Nonradioactive Probes written by Elena Hilario and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author : T. Takahashi
Publisher :
ISBN 13 :
Total Pages : 7 pages
Book Rating : 4.49/5 ( download)
Download or read book A Nonradioactive Nucleic Acid Labeling Method and a Detection Method Using it written by T. Takahashi and published by . This book was released on 1989 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Christoph Kessler
Publisher : Springer Science & Business Media
ISBN 13 : 9783540646013
Total Pages : 1170 pages
Book Rating : 4.19/5 ( download)
Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2000-03-11 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Author : Christoph Kessler
Publisher : Springer
ISBN 13 : 3662001446
Total Pages : 449 pages
Book Rating : 4.48/5 ( download)
Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 2012-12-06 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author : David William Johnson
Publisher :
ISBN 13 :
Total Pages : 176 pages
Book Rating : 4.31/5 ( download)
Download or read book Quick Blots and Nonradioactive Detection Systems written by David William Johnson and published by . This book was released on 1990 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Tim Meads
Publisher :
ISBN 13 :
Total Pages : 82 pages
Book Rating : 4.54/5 ( download)
Download or read book DNA Fingerprinting and the Use of Non Radioactive Detection Methods written by Tim Meads and published by . This book was released on 1988 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : A. Khoo
Publisher :
ISBN 13 :
Total Pages : 125 pages
Book Rating : 4.64/5 ( download)
Download or read book Non-radioactive Detection System for PCR-microsatellite Length written by A. Khoo and published by . This book was released on 1994 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detection was carried out using a streptavidin-alkaline phosphate system using a chemiliminescent method. The results were read by observing the bands on the autoradiograph. This nonradioactive method for microsatellite-length polymorphism is an alternative to the more commonly use radioactive method. [Authors' abstract].
Author : Gabriel Bela Kalmar
Publisher :
ISBN 13 :
Total Pages : 180 pages
Book Rating : 4.11/5 ( download)
Download or read book A Non-radioactive Method of Detecting Nucleic Acids written by Gabriel Bela Kalmar and published by . This book was released on 1986 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Larry J. Kricka
Publisher : Academic Press
ISBN 13 : 0323137717
Total Pages : 373 pages
Book Rating : 4.13/5 ( download)
Download or read book Nonisotopic DNA Probe Techniques written by Larry J. Kricka and published by Academic Press. This book was released on 2012-12-02 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided. This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection. Gives extensive practical information Covers major types of nonisotopic labels and procedures Presents background information for each method Provides strategies and detailed experimental procedures for labeling and detecting DNA sequences by Fluorescence Chemiluminescence Bioluminescence Colorimetry
