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Author : Phillip S. Leventhal
Publisher :
ISBN 13 :
Total Pages : 508 pages
Book Rating : 4.44/5 ( download)
Download or read book Mechanisms of Protein Kinase C Activation and Substrate Phosphorylation written by Phillip S. Leventhal and published by . This book was released on 1993 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Lodewijk V. Dekker
Publisher : Springer Science & Business Media
ISBN 13 : 9780306478635
Total Pages : 212 pages
Book Rating : 4.33/5 ( download)
Download or read book Protein Kinase C written by Lodewijk V. Dekker and published by Springer Science & Business Media. This book was released on 2004-06-17 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Kinase C is a pivotal component of the mechanism that allows a cell to respond to its changing environment. In this book, the most significant advances in recent basic research on Protein Kinase C are explained by active researchers in the field. The first seven chapters provide a comprehensive account of the fundamental structural and biochemical properties of Protein Kinase C. The remaining chapters contain overviews of the function of Protein Kinase C, both in lower organisms and in mammalian cells, the latter with a focus on immune cells and nerve cells. This book is the only recent publication devoted entirely to Protein Kinase C and forms a major point of reference for those active in the field. In addition it will appeal to those with a general interest in biochemistry, cell biology, immunology and neurobiology.
Author : Michelle Lum
Publisher :
ISBN 13 :
Total Pages : 188 pages
Book Rating : 4.74/5 ( download)
Download or read book Mechanisms of Stimulus-induced Protein Kinase C Regulation written by Michelle Lum and published by . This book was released on 2012 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Kinase C (PKC) is a family of AGC kinases (a family including the A, G, and C protein kinases) that play critical roles in regulation of cell growth and cell cycle progression, differentiation, cell survival and apoptosis, gene expression, receptor trafficking and desensitization, and cell transformation. As regulators of fundamental cellular processes, the activity of members of this family is tightly controlled. While mechanisms of activation of these proteins have been extensively characterized, the pathways underlying signal termination are less well understood. PKCs are divided into 3 classes based on structure and coactivator requirements. Classical PKC (cPKC: PKC alpha, PKC beta I, PKC beta II and PKC gamma) are activated by diacylglycerol (DAG), Ca2+ and phosphatidylserine, while novel PKC (nPKC) do not require Ca2+ and atypical PKC (aPKC) are independent of both DAG and Ca2+.^The difference in cofactor requirements for these isozymes is a result of differences in their C1 and C2 regulatory domain structures. The C1 domain is involved in DAG binding, while the C2 domain is involved in Ca2+ binding. Ligand binding by a number of growth factor and cytokine receptors leads to activation of phospholipase C and subsequent generation of DAG and inositide trisphosphate (which mobilizes intracellular Ca2+). The accumulation of DAG leads to recruitment of cPKCs and nPKCs to the plasma membrane where they are activated due to displacement of a pseudosubstrate domain from the active site. In addition to activation by physiological signals, cPKCs and nPKCs can also be activated by pharmacological agonists that bind to the same site as DAG. These agonists include phorbol esters, such as phorbol 12-myristate 13-acetate (PMA), and bryostatins, such as bryostatin-1 (bryo). Newly synthesized PKCs are not competent for activation but require priming phosphorylation on three conserved sites on the activation loop, turn motif and hydrophobic motif. These priming phosphorylations, which occur in an ordered manner, render the kinase competent for activation and stabilize it against denaturation and degradation. Acute termination of signaling is achieved by the rapid metabolism of DAG (by e. g., diacylglycerol kinase). This loss of signal leads to "reverse translocation" of membrane bound PKC to the cytoplasm in a process that is dependent on kinase activity. Pharmacological agonists are not subject to rapid metabolism and thus elicit a sustained activation of PKCs. In addition to acute termination of signal, it has long been recognized that prolonged activation of PKCs (either by pharmacological agonists or sustained physiological stimuli) leads to desensitization of this signaling system via degradation of the protein. Although activation-induced loss of PKC expression has been observed in many systems, there is considerable confusion regarding the mechanism(s) underlying agonist-induced desensitization of PKC signaling. Part of this confusion may stem from the extensive use of overexpression systems to analyze aspects of PKC regulation. While these systems can yield valuable information, our analysis indicates that overexpression can drive activated PKC isozymes into alternate pathways of degradation, which do not reflect those utilized by the endogenous proteins. Studies detailed herein analyze various mechanisms of desensitization of PKC signaling that are relevant to the endogenous protein by examining the effects of various agonists on the localization and processing of cellular PKC alpha.^The first part of this work used a combination of biochemical and immunolocalization studies to examine the mechanisms underlying a novel non-proteasomal pathway of degradation that is induced by prolonged bryo treatment in various cell types. Bryo initially induces translocation of endogenous PKC alpha; to the plasma membrane, where a pool of the protein is subjected to proteasomal degradation. A second subpopulation is internalized through a clathrin-independent, but cholesterol- and genistein-sensitive pathway, which involves trafficking through EEA1-positive early endosomes and Rab7-positive late endosomes/multivesicular bodies. The ultimate fate of internalized PKC alpha is degradation by lysosomal proteases in the perinuclear region. Analysis of the effect of endolysosomal disrupting agents in multiple cell lines points to lysosomal processing of activated PKC alpha as a common mechanism for its desensitization. The second part of this thesis explored the role of dephosphorylation and heat shock proteins (Hsps) in agonist-induced proteasomal degradation of endogenous PKC alpha. A widely accepted mechanism for stimulus-induced downregulation of PKCs involves priming site dephosphorylation, which targets the protein for proteasomal degradation. However, studies described here demonstrate that PKC agonists induce downregulation of endogenous PKCalpha with minimal accumulation of non-phosphorylated enzyme in multiple cell types. Furthermore, all or most of the non-phosphorylated enzyme detected in agonist-treated cells results from delayed maturation rather than dephosphorylation of the protein. Thus, PKC agonists induce at most low levels of dephosphorylation of endogenous PKC alpha;, and dephosphorylation is not a prerequisite for enzyme degradation. Analysis of the functions of Hsp90 and Hsp70/Hsc70 revealed distinct roles for these chaperones in regulating agonist-induced PKC alpha; degradation. Hsp90 prevents dephosphorylation of the activated enzyme and protects the mature, phosphorylated form of protein from proteasomal clearance following activation. In contrast, while Hsp70/Hsc70 also protects PKC alpha; from dephosphorylation, it enhances degradation of activated PKC alpha; by facilitating proteasomal processing of mature phosphorylated protein. Notably, downregulation of non-phosphorylated enzyme showed little dependence on Hsp70/Hsc70, suggesting that mature and non-phosphorylated species are targeted for proteasomal degradation via different pathways. Finally, lysosomal degradation of PKC alpha is not dependent on Hsps or the phosphorylation state of the enzyme. The third part of this work examines desensitization of PKC signaling following stimulation by DAG, the major physiological activator of cPKC and nPKC isozymes.^Analysis of the effects of a single addition of various DAGs (which are rapidly metabolized), and of short term pulse treatment with phorbol esters, confirmed that acute reversal of PKC alpha signaling following loss of signal involves dissociation of fully phosphorylated PKC alpha from the plasma membrane and cytosolic accumulation of the enzyme, via a mechanism that is dependent on PKC alpha activity. In contrast, repeated addition of DAGs resulted in sustained association of PKC alpha with the plasma membrane, in a pattern comparable with that induced by the phorbol ester PMA. Unlike the effects of PMA, however, chronic DAG stimulation failed to promote degradation/downregulation of PKC alpha, although downregulation of PKC delta and epsilon was readily apparent. Priming site dephosphorylation of PKC alpha was also not observed and Hsp70/Hsc70 and Hsp90 were excluded as regulators of PKC alpha phosphorylation and stability in this context. Importantly, while long-term activation of PKC alpha by DAG did not lead to degradation of the enzyme, it did induce desensitization of PKC alpha signaling, as seen by reversal of PKC alpha mediated effects on ERK activation, p21Waf1/Cip1 induction, and Id1 and cyclin D1 downregulation. These findings point to a previously undefined mechanisms for desensitization of PKC alpha, which can be attributed either to direct effects on PKC alpha function or to alterations in its downstream signaling pathways. Through analysis of the endogenous protein, each of these studies has identified a novel mechanism for desensitization of PKC alpha signaling. Collectively, the data confirm that the phosphorylated species of PKC alpha is a direct target for proteasomal and lysosomal degradation, and highlight the existence of multiple mechanisms for processing activated PKCs in cells.
Author : Alexandra C. Newton
Publisher : Springer Science & Business Media
ISBN 13 : 1592593976
Total Pages : 565 pages
Book Rating : 4.72/5 ( download)
Download or read book Protein Kinase C Protocols written by Alexandra C. Newton and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction. Protein Kinase C Protocols covers fundamental methods for studying the structure, function, regulation, subcellular localization, and macromolecular interactions of PKC. Protein Kinase C Protocols is divided into 11 sections representing the major aspects of PKC regulation and function. Part I contains an introduction and a historical perspective on the discovery of PKC by Drs. Yasutomi Nishizuka and Ushio Kikkawa. Part II describes methods to purify PKC. Part III describes the standard methods for measuring PKC activity: its enzymatic activity and its stimulus-dependent translocation from the cytosol to the membrane. Part IV describes methods for measuring the membrane interaction of PKC in vivo and in vitro. Part V provides methodologies and techniques for measuring the ph- phorylation state of PKC, including a protocol for measuring the activity of PKC’s upstream kinase, PDK-1. Novel methods for identifying substrates are described in Part VI. Part VII presents protocols for expressing and analyzing the membrane targeting domains of PKC. Part VIII provides a comprehensive c- pilation of methods used to identify binding partners for PKC. Part IX describes pharmacological probes used to study PKC. The book ends with a presentation of genetic approaches to study PKC (Part X) and a discussion of approaches used to study PKC in disease (Part XI).
Author : Michael J. Clemens
Publisher : CRC Press
ISBN 13 : 9789057020308
Total Pages : 300 pages
Book Rating : 4.00/5 ( download)
Download or read book Protein Phosphorylation in Cell Growth Regulation written by Michael J. Clemens and published by CRC Press. This book was released on 1996-12-03 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this text is to integrate the processes of protein phosphorylation and dephosphorylation into the complex pathways by which cellular proliferation is driven, bringing together the many different systems of control implicated in the regulation of cell growth. Presents a survey of protein phosphorylation roles in the control of cellular proliferation and differentiation. A large number of protein kinases and phosphatases have been characterised in higher cells, and have been shown to be involved in signal transduction pathways by which growth factors, mitogens, and extracellular agents exert proliferative effects on cells. Important subjects covered include control of gene expression at the transcriptional and translational levels, and roles of the cdk kinases and cyclins in cell cycles regulation. Describes all major families of protein kinases of significance to growth regulation.
Author : Irene Tobias
Publisher :
ISBN 13 :
Total Pages : 154 pages
Book Rating : 4.66/5 ( download)
Download or read book Molecular Mechanisms of Atypical Protein Kinase C Regulation in Insulin Signaling written by Irene Tobias and published by . This book was released on 2016 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atypical protein kinase C (aPKC) isozymes are key modulators of insulin signaling, and their dysfunction correlates with insulin-resistant states in both mice and humans. Despite the engaged interest in the importance of aPKCs to type 2 diabetes, much less is known about the molecular mechanisms that govern their cellular functions than for the conventional and novel PKC isozymes and the functionally-related Akt family of kinases. Here we show that aPKC is constitutively phosphorylated and basally active in cells. Specifically, we show that phosphorylation at two key regulatory sites, the activation loop and turn motif, of the aPKC PKC[zeta] in multiple cultured cell types is constitutive and independently regulated by separate kinases: ribosome-associated mTORC2 mediates co-translational phosphorylation of the turn motif, followed by phosphorylation at the activation loop by PDK1. Live cell imaging reveals that global aPKC activity is constitutive and insulin-unresponsive. Thus, insulin stimulation does not activate PKC[zeta] through the canonical phosphatidylinositol-3,4,5-triphosphate-mediated pathway that activates Akt, contrasting with previous literature on PKC[zeta] activation. We additionally show that protein scaffolds not only localize, but also differentially control the catalytic activity of PKC[zeta], thus promoting activity towards localized substrates and restricting activity towards global substrates. Using cellular substrate readouts and scaffolded activity reporters in live cell imaging, we show that PKC[zeta] has highly localized and differentially-controlled activity on the scaffolds p62 and Par6. Both scaffolds tether aPKC in an active conformation as assessed through pharmacological inhibition of basal activity, monitored using a genetically-encoded reporter for PKC activity. However, binding to Par6 is of higher affinity and more effective in locking PKC[zeta] in an active conformation. FRET-based translocation assays reveal that insulin promotes the association of both p62 and aPKC with the insulin-regulated scaffold IRS-1. Using the aPKC substrate MARK2 as another readout for activity, we show that overexpression of IRS-1 reduces the phosphorylation of MARK2 and enhances its plasma membrane localization, indicating sequestration of aPKC by IRS-1 away from MARK2. These results are consistent with scaffolds serving as allosteric activators of aPKCs, tethering them in an active conformation near specific substrates. Thus, signaling of these intrinsically low activity kinases is kept at a minimum in the absence of scaffolding interactions, which position the enzymes for stoichiometric phosphorylation of substrates co-localized on the same protein scaffold.
Author : Timothy R. Baffi
Publisher :
ISBN 13 :
Total Pages : 212 pages
Book Rating : 4.81/5 ( download)
Download or read book The Molecular Basis of Protein Kinase C Regulatory Mechanisms in Cancer and Neurodegenerative Disease written by Timothy R. Baffi and published by . This book was released on 2019 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein kinase C (PKC) isozymes transduce the myriad of signals downstream of phospholipid hydrolysis that potentiate an array of cellular processes including proliferation, differentiation, migration, and memory. PKC function is dysregulated in a variety of pathological states, including cancer and neurodegenerative disease. To maintain signaling fidelity, PKCs rely upon precise regulatory mechanisms that orchestrate the phosphorylations and conformational transitions that specify their signaling output. This thesis describes the molecular mechanisms by which PKC phosphorylation and autoinhibition depends upon the kinases PDK1 and mTORC2, and is opposed by PHLPP phosphatases, to produce a primed enzyme that is appropriately tuned to respond to activating signals. Specifically, we uncover the molecular basis for the controversial role of mTORC2 in AGC kinase activation by identifying a novel and conserved mTOR phosphorylation site in the C-terminal tail. Phosphorylation of this, which we term the TOR-Interaction Motif (TIM), promotes PDK1 phosphorylation of the activation loop and intramolecular autophosphorylation of the hydrophobic motif to control activation of PKC and related AGC kianse Akt. Examination of the interrelated processes of phosphorylation and autoinhibition unveils a critical role for the pseudosubstrate in protecting PKC from dephosphorylation by phosphatase PHLPP1, which selectively promotes the dephosphorylation and degradation of aberrantly active PKCs to provide a PKC quality control mechanism. High-throughput protein-level analysis from patient samples reveals that PKC quality control is a critical signaling node that sets PKC expression levels and serves as a prominent loss-of-function mechanism to impair PKC tumor-suppressive function in cancer. Critically, diseases driven by PKC dysregulation rely upon impaired PKC quality control. LOF PKC mutations in chordoid glioma act in a dominant-negative fashion to globally suppress PKC output; whereas, GOF PKC mutations in spinocerebellar ataxia drive phosphoproteome-wide changes in the cerebellum. Taken together, this thesis expands upon biochemical mechanisms of PKC maturation to identify the structural and molecular determinants of PKC phosphorylation and implicates PHLPP1 as the master regulator of PKC signaling fidelity through PKC quality control. This work is not only relevant to the pathology of disease-associated mutations in cancer and neurodegenerative disease, but also to the development of therapeutics that attempt to modulate PKC activity by targeting these regulatory mechanisms.
Author : Robert Brelsford Nelson
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.84/5 ( download)
Download or read book Protein Kinase C Substrate Phosphorylation in Relation to Neural Growth and Synaptic Plasticity written by Robert Brelsford Nelson and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jaime A. Marach
Publisher :
ISBN 13 :
Total Pages : 282 pages
Book Rating : 4.33/5 ( download)
Download or read book Processing of Protein Kinase C by Phosphorylation written by Jaime A. Marach and published by . This book was released on 2006 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Erica Monique Dutil
Publisher :
ISBN 13 :
Total Pages : 304 pages
Book Rating : 4.19/5 ( download)
Download or read book Phosphorylation of Protein Kinase C by the Phosphoinositide-dependent Kinase written by Erica Monique Dutil and published by . This book was released on 2000 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
