Low Cost Methods For Molecular Characterization Of Mutant Plants

Author : Bradley J. Till
Publisher : Springer
ISBN 13 : 3319162594
Total Pages : 35 pages
Book Rating : 4.91/5 ( download)

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Download or read book Low-Cost Methods for Molecular Characterization of Mutant Plants written by Bradley J. Till and published by Springer. This book was released on 2015-05-08 with total page 35 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Author : Joanna Jankowicz-Cieslak
Publisher : Springer
ISBN 13 : 3319450212
Total Pages : 343 pages
Book Rating : 4.16/5 ( download)

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Download or read book Biotechnologies for Plant Mutation Breeding written by Joanna Jankowicz-Cieslak and published by Springer. This book was released on 2016-12-08 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Author : Tariq Ahmad Bhat
Publisher : CRC Press
ISBN 13 : 1000613968
Total Pages : 308 pages
Book Rating : 4.64/5 ( download)

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Download or read book Biotechnologies and Genetics in Plant Mutation Breeding written by Tariq Ahmad Bhat and published by CRC Press. This book was released on 2023-06-30 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: "An indispensable source for researchers, teachers, and graduate and postgraduate students interested in mutation breeding and genetic engineering. It introduces readers to contemporary knowledge and state-of-the-art technologies in the field of mutation breeding, including fundamental mechanisms and applications. . . . It will provide new directions, and avenues for enhancement of food security and food quality by using the latest techniques for the 'mutation as breeding' approach." - From Prof. Jameel M. Al-Khayri, King Faisal University, Saudi Arabia This comprehensive three-volume set book aims to help combat the challenge of providing enough food for the world by the use of advanced genetic processes to improve crop production, both in quantity and quality. Volume 1: Mutagenesis and Crop Improvement discusses mutagenesis, cytotoxicity, and crop improvement, covering the processes, mutagenic effectiveness, and mechanisms. The volume emphasizes the improvement of agronomic characteristics by manipulating the genotype of plant species, resulting in increased productivity. Volume 2: Revolutionizing Plant Biology covers the use of mutagenesis and biotechnology to explore the variability of mutant genes for crop improvement. The chapters deal with in-vitro mutagenesis to exploit the somaclonal variations induced in cell culture and highlight the importance of in-vitro mutagenesis in inducing salt resistance, heat resistance, and drought resistance. Volume 3: Mechanisms for Genetic Manipulation of Plants and Plant Mutants reviews the genetic engineering techniques used to mutate genes and to incorporate them into different plant species of cereals, pulses, vegetables, and fruits. Also discussed are the principles of genetic engineering by which desired genes can be transferred from plants to animals to microorganisms and vice versa.

Author : Dr. Inamuddin
Publisher : Elsevier
ISBN 13 : 0323951570
Total Pages : 474 pages
Book Rating : 4.79/5 ( download)

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Download or read book Green Sustainable Process for Chemical and Environmental Engineering and Science written by Dr. Inamuddin and published by Elsevier. This book was released on 2023-01-11 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Green Sustainable Process for Chemical and Environmental Engineering and Science: Green Solvents and Extraction Technology provides information on the use of green solvents and their applications in the synthesis of pharmaceutical drugs, energy conversion and storage, catalysis, biodiesel synthesis, multicomponent reactions, waste valorization, and more. The book features introductory chapters related to the applications of green solvents and related extraction technology for sustainable development, including research trends, technical development, environment issues, and related concerns. The book provides examples covering the extraction of nanocellulose (from agricultural wastes), polysaccharides, phenolic compounds, antioxidants (from vegetables), biomolecules and green solvents (from biomass and precious metals). Provides an overview of the applicability of green solvents for sustainable development Delivers in-depth literature on the use of green solvents for industrial processes Highlights issues related to research trends, sustainable development, and the environment Focuses on extraction technology Offers an overview of the use of green solvent-based extraction Presents in-depth literature on the extraction of a variety of substances using green solvents

Author : Q. Y. Shu
Publisher : CABI
ISBN 13 : 1780640854
Total Pages : 612 pages
Book Rating : 4.53/5 ( download)

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Download or read book Plant Mutation Breeding and Biotechnology written by Q. Y. Shu and published by CABI. This book was released on 2012 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.

Author : A. Karp
Publisher : Bioversity International
ISBN 13 : 929043323X
Total Pages : 47 pages
Book Rating : 4.31/5 ( download)

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Download or read book Molecular Tools in Plant Genetic Resources Conservation written by A. Karp and published by Bioversity International. This book was released on 1997 with total page 47 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of a workshop on the use of molecular techniques in the conservation of plant genetic resources.

Author : Rajeev K. Varshney
Publisher : Springer
ISBN 13 : 3319913131
Total Pages : 298 pages
Book Rating : 4.31/5 ( download)

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Download or read book Plant Genetics and Molecular Biology written by Rajeev K. Varshney and published by Springer. This book was released on 2018-09-04 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the latest advances in multiple fields of plant biotechnology and the opportunities that plant genetics, genomics and molecular biology have offered for agriculture improvement. Advanced technologies can dramatically enhance our capacity in understanding the molecular basis of traits and utilizing the available resources for accelerated development of high yielding, nutritious, input-use efficient and climate-smart crop varieties. In this book, readers will discover the significant advances in plant genetics, structural and functional genomics, trait and gene discovery, transcriptomics, proteomics, metabolomics, epigenomics, nanotechnology and analytical & decision support tools in breeding. This book appeals to researchers, academics and other stakeholders of global agriculture.

Author : Khalid Meksem
Publisher : John Wiley & Sons
ISBN 13 : 9783527629404
Total Pages : 460 pages
Book Rating : 4.08/5 ( download)

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Download or read book The Handbook of Plant Mutation Screening written by Khalid Meksem and published by John Wiley & Sons. This book was released on 2009-12-21 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Induced mutagenesis is a common and promising method for screening for new crops with improved properties. This title introduces the different methods and then focuses on the screening, detection and analysis of the novel mutations. Written by a global team of authors the book is an indispensable tool for all scientists working on crop breeding in industry and academia.

Author : Jameel M. Al-Khayri
Publisher : Springer
ISBN 13 : 3319225219
Total Pages : 656 pages
Book Rating : 4.10/5 ( download)

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Download or read book Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools written by Jameel M. Al-Khayri and published by Springer. This book was released on 2016-02-02 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basic concept of this book is to examine the use of innovative methods augmenting traditional plant breeding towards the development of new crop varieties under different environmental conditions to achieve sustainable food production. This book consists of two volumes: Volume 1 subtitled Breeding, Biotechnology and Molecular Tools and Volume 2 subtitled Agronomic, Abiotic and Biotic Stress Traits. This is Volume 1 which consists of 21 chapters covering domestication and germplasm utilization, conventional breeding techniques and the role of biotechnology. In addition to various biotechnological applications in plant breeding, it includes functional genomics, mutations and methods of detection, and molecular markers. In vitro techniques and their applications in plant breeding are discussed with an emphasis on embryo rescue, somatic cell hybridization and somaclonal variation. Other chapters cover haploid breeding, transgenics, cryogenics and bioinformatics.

Author : H S Chawla
Publisher : CRC Press
ISBN 13 : 1439894140
Total Pages : 745 pages
Book Rating : 4.49/5 ( download)

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Download or read book Introduction to Plant Biotechnology (3/e) written by H S Chawla and published by CRC Press. This book was released on 2011-05-24 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.