Looking At Ribozymes

Author : Benoît Masquida
Publisher : John Wiley & Sons
ISBN 13 : 1394276621
Total Pages : 196 pages
Book Rating : 4.22/5 ( download)

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Download or read book Looking at Ribozymes written by Benoît Masquida and published by John Wiley & Sons. This book was released on 2024-02-21 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Behind the neologism “ribozymes” lies a family of fascinating molecules, ribo-enzymes, which have been relatively little studied. These catalytically active RNAs are found in all strata of life, from viruses to the human genome. At the end of the 1970s, the discovery of a catalytic RNA nestled in an intron, followed by another involved in the maturation of transfer RNAs, led to the discovery of new ribozymes and the transition from a strictly “proteocentric” vision, inherited from the dogma of molecular biology, to a more “nucleocentric” one. Since then, a variety of ribozymes have been identified in genomes, where their functions often remain mysterious. Looking at Ribozymes traces the discovery of these molecules and presents a picture of their functional diversity, catalytic mechanisms and distribution within the tree of life.

Author : Benoît Masquida
Publisher : Elsevier
ISBN 13 : 0081019599
Total Pages : 130 pages
Book Rating : 4.97/5 ( download)

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Download or read book Looking at Ribozymes written by Benoît Masquida and published by Elsevier. This book was released on 2017-10-01 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book introduces some key ideas about how ribozymes “work through a comparison of their common features and specificities. “How do they work ? is not just a matter of enzymatic catalysis but also, how the (macro) molecular organization at different scales makes it possible, what function they carry, where they are found and how they evolved, and how they have been used or designed for other purposes. The book emphasizes the common and specific features of natural and artificial ribozymes from the basics to the more advanced aspects of current knowledge. It covers different areas of research, from the atomic scale (catalysis) to the molecular (folding, dynamics), macromolecular and cellular scales (dynamics, long-range interactions, self-assembly, cellular processes). It is not a directory of ribozymes but provides comparative knowledge including the inter-dependent relationships between the different scales of description based on different experimental and theoretical approaches. It is intended to provide a new way of looking at ribozymes that can be a source of inspiration for undergraduate, graduate students and researchers working in the field or interested in ribozymes. Comparative knowledge of ribozymes based on scales of organization and scales of description

Author : Sabine Müller
Publisher : John Wiley & Sons
ISBN 13 : 3527814531
Total Pages : 81 pages
Book Rating : 4.34/5 ( download)

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Download or read book Ribozymes written by Sabine Müller and published by John Wiley & Sons. This book was released on 2021-07-09 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

Author : David Malcolm James Lilley
Publisher : Royal Society of Chemistry
ISBN 13 : 0854042539
Total Pages : 339 pages
Book Rating : 4.31/5 ( download)

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Download or read book Ribozymes and RNA Catalysis written by David Malcolm James Lilley and published by Royal Society of Chemistry. This book was released on 2008 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Takes the reader through the origins of catalysis in RNA and necessarily includes significant discussion of structure and folding. The main focus of the book concerns chemical mechanism with extensive comment on how, despite the importance of RNA catalysis in the cell, its origins are still poorly understood and often controversial. The reader is given an outline of the important role of RNA catalysis in many aspects of cell function, including RNA processing and translation.

Author : John J. Rossi
Publisher : Taylor & Francis
ISBN 13 : 9781898486176
Total Pages : 294 pages
Book Rating : 4.74/5 ( download)

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Download or read book Intracellular Ribozyme Applications written by John J. Rossi and published by Taylor & Francis. This book was released on 1999 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this definitive guide to intracellular ribozyme applications, researchers from both industry and academia review the most recent pharmaceutical, therapeutic, and biotechnological applications of ribozymes. Leading scientists from around the world present the latest principles on the application of ribozyme technology to the inhibition of gene expression in living cells. The authors are experts in the field and describe, in detail, protocols for monitoring ribozyme activity, for expressing ribozymes from DNA vectors, for delivering ribozymes to cells, and for in vitro selection of improved ribozymes. The book provides a forward-looking view of ribozyme technology for alteration of gene expression in plants and in treatment of retroviral and genetic diseases in man.

Author : Ian Gibson
Publisher : John Wiley & Sons
ISBN 13 : 3527612521
Total Pages : 92 pages
Book Rating : 4.29/5 ( download)

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Download or read book Antisense and Ribozyme Methodology written by Ian Gibson and published by John Wiley & Sons. This book was released on 2008-09-26 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating viral infections and cancer. This laboratory companion provides detailed information on the potential, advantages and limitations of this methodology. It critically discusses potential pitfalls, presents strategies for choosing targets and delivery systems, so as to allow the selection of the optimum methodology for achieving fast and reliable experimental success with any human or other biological system. For researchers, technicians and advanced graduates in experimental medicine, molecular and cell biology.

Author : Philip C. Turner
Publisher : Humana
ISBN 13 : 9781489942852
Total Pages : 492 pages
Book Rating : 4.58/5 ( download)

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Download or read book Ribozyme Protocols written by Philip C. Turner and published by Humana. This book was released on 2013-09-20 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use—^not only to those with some experience of ribozymes—^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field, I believe this book approaches that ideal and should help all readers perform meaningful experiments using ribozymes. To design ribozymes, one must consider whether the target site will be accessible; this task can be facilitated by using computer programs that pre dict the folding of the target RNA. Such programs are detailed in Chapters 2 and 3. If the chosen target is an RNA virus that can mutate rapidly, it makes sense to consider those parts of the genome that are least likely to change during viral replication. An example of how this can be done is described in Chapter 4. Although computer analysis may be a useful starting point to select tar get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select ing those that actually hybridize to and/or cleave the target; these methods are described in Chapters 5 and 6.

Author : Robert J Scarborough
Publisher : Humana
ISBN 13 : 9781071607183
Total Pages : 304 pages
Book Rating : 4.89/5 ( download)

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Download or read book Ribozymes written by Robert J Scarborough and published by Humana. This book was released on 2021-08-09 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide RNAs, AgoshRNAs and aptamers or to study RNA capping and long non-coding RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ribozymes: Methods and Protocols aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function.

Author : Kevin J. Scanlon
Publisher : Humana
ISBN 13 : 9781489943439
Total Pages : 462 pages
Book Rating : 4.39/5 ( download)

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Download or read book Therapeutic Applications of Ribozymes written by Kevin J. Scanlon and published by Humana. This book was released on 2013-08-08 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of Applications Therapeutic of Ribozymes is to provide an overview of the utility of ribozymes to selectively inhibit the expression of RNA. The ribozyme applications appearing in this book should benefit not only those experienced in ribozymes, but also those applying this ribozyme technology for the first time. It is hoped that a better understanding of the therapeutic application ofribozymes will have a significant impact on human disease in the near future. The field ofribozyme biochemistry has come a long way since the initial observation that RNA is capable of catalysis, initially in cis during processing of larger molecules and ultimately the use of ribozymes in trans to achieve cleavage of target sequence. The fundamental observation that hammerhead (and subsequently hairpin) ribozymes could be designed to cleave a target messenger RNA, and the clarification of sequence restrictions in both the ribozyme and the target RNA, have paved the ways for the use of ribozymes as a tool to manipulate gene expression at the molecular level. This had pre- ously been a domain of antisense oligonucleotides and triplex DNA. Sub- quent studies have explored the myriad biological systems in which ribozyme-mediated inhibition of genes involved in various cellular processes may be used to uncouple important signaling pathways or to reverse the p- notypic expression of a pathologic process.

Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.81/5 ( download)

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Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: