Lc Ms

Author : Marvin C. McMaster
Publisher : John Wiley & Sons
ISBN 13 : 0471736570
Total Pages : 184 pages
Book Rating : 4.78/5 ( download)

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Download or read book LC/MS written by Marvin C. McMaster and published by John Wiley & Sons. This book was released on 2005-08-08 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practical guide to using and maintaining an LC/MS system The combination of liquid chromatography (LC) and mass spectrometry(MS) has become the laboratory tool of choice for a broad range ofindustries that require the separation, analysis, and purificationof mixtures of organic compounds. LC/MS: A Practical User's Guide provides LC/MS users with aneasy-to-use, hands-on reference that focuses on the practicalapplications of LC/MS and introduces the equipment and techniquesneeded to use LC/MS successfully. Following a thorough explanationof the basic components and operation of the LC/MS system, theauthor presents empirical methods for optimizing the techniques,maintaining the instrumentation, and choosing the appropriate MS orLC/MS analyzer for any given problem. LC/MS covers everything users need to know about: The latest equipment, including quadrupole, time-of-flight, andion trap analyzers Cutting-edge processes, such as preparing HPLC mobile phasesand samples; handling and maintaining a wide variety of silica,zirconium, and polymeric separation columns; interpreting andquantifying mass spectral data; and using MS interfaces Current and future applications in the pharmaceutical andagrochemical industries, biotechnology, clinical research,environmental studies, and forensics An accompanying PowerPoint® slide-set on CD-ROM provides vitalteaching tools for instructors and new equipment operators.Abundantly illustrated and easily accessible, the text is designedto help students and practitioners acquire optimum proficiency inthis powerful and rapidly advancing analytical application.

Author : Lloyd R. Snyder
Publisher : John Wiley & Sons
ISBN 13 : 1118210395
Total Pages : 831 pages
Book Rating : 4.90/5 ( download)

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Download or read book Introduction to Modern Liquid Chromatography written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Author : Loralie J. Langman
Publisher : Humana Press
ISBN 13 : 9781617799334
Total Pages : 0 pages
Book Rating : 4.35/5 ( download)

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Download or read book LC-MS in Drug Analysis written by Loralie J. Langman and published by Humana Press. This book was released on 2012-07-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Author : Q. Alan Xu
Publisher : Springer Science & Business Media
ISBN 13 : 1461438284
Total Pages : 476 pages
Book Rating : 4.81/5 ( download)

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Download or read book LC-MS in Drug Bioanalysis written by Q. Alan Xu and published by Springer Science & Business Media. This book was released on 2012-07-15 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical pharmacology plays an important role in today’s medicine. Due to the high sensitivity, selectivity, and affordability of a mass spectrometer (MS), the high performance liquid chromatography – mass spectrometry (LC-MS) analytical technique is widely used in the determination of drugs in human biological matrixes for clinical pharmacology. Specifically, LC-MS is used to analyze: anticancer drugs antidementia drugs antidepressant drugs antiepileptic drugs antifundal drug antimicrobial drugs antipsychotic drugs antiretroviral drugs anxiolytic/hypnotic drugs cardiac drugs drugs for addiction immunosuppressant drugs mood stabilizer drugs This book will primarily cover the various methods of validation for LC-MS techniques and applications used in modern clinical pharmacology.

Author : Wenkui Li
Publisher : John Wiley & Sons
ISBN 13 : 111867135X
Total Pages : 709 pages
Book Rating : 4.51/5 ( download)

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Download or read book Handbook of LC-MS Bioanalysis written by Wenkui Li and published by John Wiley & Sons. This book was released on 2013-09-03 with total page 709 pages. Available in PDF, EPUB and Kindle. Book excerpt: Consolidates the information LC-MS bioanalytical scientists need to analyze small molecules and macromolecules The field of bioanalysis has advanced rapidly, propelled by new approaches for developing bioanalytical methods, new liquid chromatographic (LC) techniques, and new mass spectrometric (MS) instruments. Moreover, there are a host of guidelines and regulations designed to ensure the quality of bioanalytical results. Presenting the best practices, experimental protocols, and the latest understanding of regulations, this book offers a comprehensive review of LC-MS bioanalysis of small molecules and macromolecules. It not only addresses the needs of bioanalytical scientists working on routine projects, but also explores advanced and emerging technologies such as high-resolution mass spectrometry and dried blood spot microsampling. Handbook of LC-MS Bioanalysis features contributions from an international team of leading bioanalytical scientists. Their contributions reflect a review of the latest findings, practices, and regulations as well as their own firsthand analytical laboratory experience. The book thoroughly examines: Fundamentals of LC-MS bioanalysis in drug discovery, drug development, and therapeutic drug monitoring The current understanding of regulations governing LC-MS bioanalysis Best practices and detailed technical instructions for LC-MS bioanalysis method development, validation, and stability assessment of analyte(s) of interest Experimental guidelines and protocols for quantitative LC-MS bioanalysis of challenging molecules, including pro-drugs, acyl glucuronides, N-oxides, reactive compounds, and photosensitive and autooxidative compounds With its focus on current bioanalytical practice, Handbook of LC-MS Bioanalysis enables bioanalytical scientists to develop and validate robust LC-MS assay methods, all in compliance with current regulations and standards.

Author : Wenkui Li
Publisher : John Wiley & Sons
ISBN 13 : 111927429X
Total Pages : 384 pages
Book Rating : 4.92/5 ( download)

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Download or read book Sample Preparation in LC-MS Bioanalysis written by Wenkui Li and published by John Wiley & Sons. This book was released on 2019-03-12 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes) Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor) Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates) Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.

Author : Thomas Letzel
Publisher : Royal Society of Chemistry
ISBN 13 : 1849733147
Total Pages : 352 pages
Book Rating : 4.44/5 ( download)

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Download or read book Protein and Peptide Analysis by LC-MS written by Thomas Letzel and published by Royal Society of Chemistry. This book was released on 2011-07-22 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.

Author : Robert E. Ardrey
Publisher : John Wiley & Sons
ISBN 13 : 0470862173
Total Pages : 296 pages
Book Rating : 4.79/5 ( download)

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Download or read book Liquid Chromatography - Mass Spectrometry written by Robert E. Ardrey and published by John Wiley & Sons. This book was released on 2003-07-25 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: First explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique. * First book to concentrate on principles of LC-MS * Explains principles of mass spectrometry and chromatography before moving on to LC-MS * Describes instrumental aspects of LC-MS * Discusses current applications of LC-MS and shows benefits of using this technique in practice

Author : Aldo Polettini
Publisher : Pharmaceutical Press
ISBN 13 : 9780853696292
Total Pages : 310 pages
Book Rating : 4.92/5 ( download)

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Download or read book Applications of LC-MS in Toxicology written by Aldo Polettini and published by Pharmaceutical Press. This book was released on 2006 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical toxicologists are involved in the analysis of drugs and poisons in biological samples in different environments. Many scientists in the field of analytical toxicology have adopted LC-MS in their daily work, and this is illustrated by the increasing numbers of research papers published and presented at relevant conferences.

Author : Pedro R. Cutillas
Publisher : Humana Press
ISBN 13 : 9781607617815
Total Pages : 330 pages
Book Rating : 4.11/5 ( download)

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Download or read book LC-MS/MS in Proteomics written by Pedro R. Cutillas and published by Humana Press. This book was released on 2011-04-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.