Label Free Super Resolution Microscopy

Author : Vasily Astratov
Publisher : Springer Nature
ISBN 13 : 3030217221
Total Pages : 487 pages
Book Rating : 4.28/5 ( download)

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Download or read book Label-Free Super-Resolution Microscopy written by Vasily Astratov and published by Springer Nature. This book was released on 2019-08-31 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Author : Ji-Xin Cheng
Publisher : Elsevier
ISBN 13 : 0323903371
Total Pages : 612 pages
Book Rating : 4.70/5 ( download)

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Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics

Author : Anda Cornea
Publisher : Elsevier
ISBN 13 : 0124167136
Total Pages : 261 pages
Book Rating : 4.31/5 ( download)

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Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Author : Udo J. Birk
Publisher : John Wiley & Sons
ISBN 13 : 3527802061
Total Pages : 260 pages
Book Rating : 4.67/5 ( download)

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Download or read book Super-Resolution Microscopy written by Udo J. Birk and published by John Wiley & Sons. This book was released on 2017-07-21 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Author : R. Rigler
Publisher : Springer Science & Business Media
ISBN 13 : 3642565441
Total Pages : 375 pages
Book Rating : 4.41/5 ( download)

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Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Author : Alberto Diaspro
Publisher : CRC Press
ISBN 13 : 1315355566
Total Pages : 525 pages
Book Rating : 4.66/5 ( download)

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Download or read book Super-Resolution Imaging in Biomedicine written by Alberto Diaspro and published by CRC Press. This book was released on 2016-11-03 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Author : Thomas Muller-Reichert
Publisher : Academic Press
ISBN 13 : 0124160263
Total Pages : 466 pages
Book Rating : 4.62/5 ( download)

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Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

Author : Philip Tinnefeld
Publisher : Springer
ISBN 13 : 3662455471
Total Pages : 340 pages
Book Rating : 4.70/5 ( download)

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Download or read book Far-Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Author : Jiang Wu
Publisher : John Wiley & Sons
ISBN 13 : 3527835199
Total Pages : 293 pages
Book Rating : 4.95/5 ( download)

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Download or read book Optical Imaging and Sensing written by Jiang Wu and published by John Wiley & Sons. This book was released on 2023-08-03 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical Imaging and Sensing Understand the future of optical imaging with this cutting-edge guide Optoelectronic devices for imaging and sensing are among the backbones of modern technology. Facilitating the mutual conversion of optical and electrical signals, they have applications from telecommunications to molecular spectroscopy, and their incorporation into photon-involved technologies is only growing. The rapid development of this field makes the need for a fully up-to-date introduction all the more critical. Optical Imaging and Sensing meets this need with a comprehensive guide to the novel materials and devices employed in optical imaging and sensing. Given the current revolution in new imaging materials, an introduction that fully incorporates the latest research is an indispensable tool for scientists and engineers in a huge range of fields. The technologies surveyed here promise to transform public security, 5G and next-generation wireless communication, clinical imaging, and many more. Optical Imaging and Sensing Readers will also find: Detailed discussion of materials including semimetallic graphene, semiconducting black phosphorous, and many more Discussion of devices from infrared photodetectors to nonlinear interferometers A thorough look forward to the future of the field Optical Imaging and Sensing is a useful reference for materials scientists, spectroscopists, semiconductor physicists, and engineers working in any field or industry involving optical imaging or sensing technology.

Author : P. Bianchini
Publisher : IOS Press
ISBN 13 : 1643684159
Total Pages : 176 pages
Book Rating : 4.54/5 ( download)

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Download or read book Multimodal and Nanoscale Optical Microscopy written by P. Bianchini and published by IOS Press. This book was released on 2023-09-11 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical microscopy is developing into nanoscopy and multimodal microscopy the better to decipher the functioning mechanisms in living systems, and investigating biological specimens at molecular level using fluorescence as a mechanism of contrast. Results have demonstrated the potential to provide information at the Angstrom level. Other optical methods now offer more in terms of spatial and temporal resolution, making it possible to study the delicate and complex relationship between structure and function in cells. Modern optical microscopes also use the decisive advantage provided by artificial intelligence algorithms. All in all, a rapidly changing field with an increasing number of questions to be answered. This book contains the lectures presented as Course 210 of the prestigious International School of Physics Enrico Fermi, Multimodal and Nanoscale Optical Microscopy, held in Varenna, Italy from 11 to 16 July 2021. Topics covered include fluorescence; linear and nonlinear microscopy; label-free with Mueller matrix and Brillouin microscopy; F-methods such as FRAP, FLIM, FRET and FCS; super-resolution, phototoxicity and photodamage; optical and magnetic trapping; image formation; and bioimage analysis, among others. The book also includes selected contributions of a number of young researchers. Packed with novel ideas, new instruments, challenging theoretical approaches and amazing applications, the book will be of interest to all those working in the field of multimodal and nanoscale optical microscopy.