High Dimensional Single Cell Analysis

Author : Harris G. Fienberg
Publisher : Springer
ISBN 13 : 364254827X
Total Pages : 224 pages
Book Rating : 4.77/5 ( download)

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Download or read book High-Dimensional Single Cell Analysis written by Harris G. Fienberg and published by Springer. This book was released on 2014-04-22 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.

Author : Aik T. Ooi
Publisher :
ISBN 13 : 9781071617717
Total Pages : 311 pages
Book Rating : 4.10/5 ( download)

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Download or read book Single-Cell Protein Analysis written by Aik T. Ooi and published by . This book was released on 2021 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.

Author : Xiangdong Wang
Publisher : Springer
ISBN 13 : 9401797536
Total Pages : 177 pages
Book Rating : 4.35/5 ( download)

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Download or read book Single Cell Sequencing and Systems Immunology written by Xiangdong Wang and published by Springer. This book was released on 2015-03-27 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.

Author : Karsten Kuritz
Publisher : Logos Verlag Berlin GmbH
ISBN 13 : 383255209X
Total Pages : 150 pages
Book Rating : 4.91/5 ( download)

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Download or read book Analysis and Control of Cellular Ensembles. Exploiting dimensionality reduction in single-cell data and models written by Karsten Kuritz and published by Logos Verlag Berlin GmbH. This book was released on 2020-11-20 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: An ensemble system is a collection of nearly identical dynamical systems which admit a certain degree of heterogeneity, and which are subject to the restriction that they may only be manipulated or observed as a whole. This thesis presents analysis and control methods for cellular ensembles by considering reduced 1-dimensional dynamics of biological processes in high-dimensional single-cell data and models. To be more specific, we address the quest for real-time analysis of biological processes within single-cell data by introducing the measure-preserving map of pseudotime into real-time, in short MAPiT. MAPiT enables the reconstruction of temporal and spatial dynamics from single-cell snapshot experiments. In addition, we propose a PDE-constrained learning algorithm which allows for efficient inference of changes in cell cycle progression from time series single-cell snapshot data. The second part of this thesis, is devoted to controlling a heterogeneous cell population, in the sense, that we aim at achieving a desired distribution of cellular oscillators on their periodic orbit. A systems theoretic approach to the ensemble control problem provides novel necessary and sufficient conditions for the control of phase distributions in terms of the Fourier coefficients of the phase response curve. This thesis establishes a connection between the previously separate areas of single cell analysis and ensemble control. Our holistic view opens new perspectives for theoretic concepts in basic research and therapeutic strategies in precision medicine.

Author : Emanuela Marcenaro
Publisher : Frontiers Media SA
ISBN 13 : 2889453502
Total Pages : 245 pages
Book Rating : 4.04/5 ( download)

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Download or read book NK Cell Subsets in Health and Disease: New Developments written by Emanuela Marcenaro and published by Frontiers Media SA. This book was released on 2017-11-29 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Natural Killer (NK) cells were discovered ca 1975, as the first group of lymphoid cells that were neither T cells nor B cells. Since then, the dissection of the biology of NK cells has been growing exponentially with many seminal discoveries from the identification of MHC class I-specific inhibitory receptors to the discovery of receptor-ligand pairs involved in NK cell activation and to the manipulation of NK cells in cancer. In this research topic, we asked a group of thought leaders in NK cell biology to review recent advances in their origins and biology, and their roles in cancer, infection and inflammation. Together, these 25 articles provide a timely survey of NK cells as critical immunologic components of health and disease. They will hopefully prompt further dialogue and developments in basic and translational immunology.

Author : Xinkun Wang
Publisher : CRC Press
ISBN 13 : 1000897192
Total Pages : 435 pages
Book Rating : 4.97/5 ( download)

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Download or read book Next-Generation Sequencing Data Analysis written by Xinkun Wang and published by CRC Press. This book was released on 2023-07-06 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA-seq: both bulk and single-cell (separate chapters) Genotyping and variant discovery through whole genome/exome sequencing Clinical sequencing and detection of actionable variants De novo genome assembly ChIP-seq to map protein-DNA interactions Epigenomics through DNA methylation sequencing Metagenome sequencing for microbiome analysis

Author : J Philip McCoy Jr
Publisher : Humana
ISBN 13 : 9781493996490
Total Pages : 375 pages
Book Rating : 4.95/5 ( download)

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Download or read book Immunophenotyping written by J Philip McCoy Jr and published by Humana. This book was released on 2019-09-29 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.

Author : Fan-Gang Tseng
Publisher : Springer
ISBN 13 : 3662491184
Total Pages : 415 pages
Book Rating : 4.88/5 ( download)

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Download or read book Essentials of Single-Cell Analysis written by Fan-Gang Tseng and published by Springer. This book was released on 2016-01-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Author : John Shawe-Taylor
Publisher : Cambridge University Press
ISBN 13 : 9780521813976
Total Pages : 520 pages
Book Rating : 4.72/5 ( download)

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Download or read book Kernel Methods for Pattern Analysis written by John Shawe-Taylor and published by Cambridge University Press. This book was released on 2004-06-28 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher Description

Author : Masahiro Ono
Publisher : Springer Nature
ISBN 13 : 1071626477
Total Pages : 276 pages
Book Rating : 4.74/5 ( download)

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Download or read book Regulatory T-Cells written by Masahiro Ono and published by Springer Nature. This book was released on 2022-09-30 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Regulatory T-Cells: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.