Handbook Of Biomedical Nonlinear Optical Microscopy

Author : Barry R. Masters
Publisher : Oxford University Press
ISBN 13 : 9780198036821
Total Pages : 896 pages
Book Rating : 4.25/5 ( download)

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Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Author : David A. Boas
Publisher : CRC Press
ISBN 13 : 1420090372
Total Pages : 816 pages
Book Rating : 4.76/5 ( download)

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Download or read book Handbook of Biomedical Optics written by David A. Boas and published by CRC Press. This book was released on 2016-04-19 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamental

Author : Eric Olaf Potma
Publisher : John Wiley & Sons
ISBN 13 : 1119814901
Total Pages : 453 pages
Book Rating : 4.00/5 ( download)

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Download or read book Foundations of Nonlinear Optical Microscopy written by Eric Olaf Potma and published by John Wiley & Sons. This book was released on 2024-03-13 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foundations of Nonlinear Optical Microscopy Concise yet comprehensive resource presenting the foundations of nonlinear optical microscopy Foundations of Nonlinear Optical Microscopy brings together all relevant principles of nonlinear optical (NLO) microscopy, presenting NLO microscopy within a consistent framework to allow for the origin of the signals and the interrelation between different NLO techniques to be understood. The text provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, sensitivity, and imaging contrast. The book also addresses typical questions students ask, and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead. Foundations of Nonlinear Optical Microscopy covers sample topics such as: Light propagation, focusing of light, pulses of light, classical description of light-matter interactions, and quantum mechanical description of light-matter interactions Molecular transitions, selection rules, signal radiation, and detection of light Multi-photon fluorescence and pump-probe microscopy Harmonic generation, sum-frequency generation, and coherent Raman scattering Senior undergraduate and graduate students in chemistry, physics, and biomedical engineering, along with students of electrical engineering and instructors in both of these fields, can use the information within Foundations of Nonlinear Optical Microscopy and the included learning resources to gain a concise yet comprehensive overview of the subject.

Author : Barry R. Masters
Publisher : Springer Nature
ISBN 13 : 3030216918
Total Pages : 415 pages
Book Rating : 4.17/5 ( download)

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Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Author : John Girkin
Publisher : CRC Press
ISBN 13 : 1351630350
Total Pages : 247 pages
Book Rating : 4.51/5 ( download)

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Download or read book A Practical Guide to Optical Microscopy written by John Girkin and published by CRC Press. This book was released on 2019-06-14 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Author : James G. Fujimoto
Publisher : Oxford University Press
ISBN 13 : 9780199722297
Total Pages : 440 pages
Book Rating : 4.93/5 ( download)

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Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

Author : Jerome Mertz
Publisher : Cambridge University Press
ISBN 13 : 1108428304
Total Pages : 475 pages
Book Rating : 4.09/5 ( download)

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Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Author : Kazuyoshi Itoh
Publisher : John Wiley & Sons
ISBN 13 : 1118751779
Total Pages : 286 pages
Book Rating : 4.70/5 ( download)

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Download or read book Functional Imaging by Controlled Nonlinear Optical Phenomena written by Kazuyoshi Itoh and published by John Wiley & Sons. This book was released on 2013-09-03 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.

Author : Jerome Mertz
Publisher : Cambridge University Press
ISBN 13 : 1108588360
Total Pages : 475 pages
Book Rating : 4.62/5 ( download)

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Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08-01 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.

Author : Irving J. Bigio
Publisher : Cambridge University Press
ISBN 13 : 0521876567
Total Pages : 703 pages
Book Rating : 4.68/5 ( download)

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Download or read book Quantitative Biomedical Optics written by Irving J. Bigio and published by Cambridge University Press. This book was released on 2016-01-07 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: Based on physical science principles, Quantitative Biomedical Optics covers theory, instrumentation, methods and applications, with practical exercises and problem sets.