Gene Silencing

Author : Gregory J. Hannon
Publisher : CSHL Press
ISBN 13 : 9780879696412
Total Pages : 568 pages
Book Rating : 4.19/5 ( download)

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Download or read book RNAi written by Gregory J. Hannon and published by CSHL Press. This book was released on 2003 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.

Author : Muhammad Sohail
Publisher : CRC Press
ISBN 13 : 020348925X
Total Pages : 398 pages
Book Rating : 4.53/5 ( download)

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Download or read book Gene Silencing by RNA Interference written by Muhammad Sohail and published by CRC Press. This book was released on 2004-08-27 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r

Author : Renato Baserga
Publisher :
ISBN 13 :
Total Pages : 372 pages
Book Rating : 4.59/5 ( download)

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Download or read book Antisense Strategies written by Renato Baserga and published by . This book was released on 1992 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: The antisense strategy has been used to study cellular proliferation and differentiation as well as to target chemotherapy against viral products such as HIV. It shows promise in the isolation of bone marrow stem cells and the purging of bone marrow. This volume, covering cellular proliferation, inhibition of viral replication, ribozymes-transcriptural regulation, cellular differentiation, and transgenic animals should be important to both basic and clinical scientists.

Author : Ute Schepers
Publisher : John Wiley & Sons
ISBN 13 : 3527604375
Total Pages : 336 pages
Book Rating : 4.71/5 ( download)

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Download or read book RNA Interference in Practice written by Ute Schepers and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.

Author : Tamas Dalmay
Publisher : CABI
ISBN 13 : 1780647670
Total Pages : 223 pages
Book Rating : 4.78/5 ( download)

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Download or read book Plant Gene Silencing written by Tamas Dalmay and published by CABI. This book was released on 2017-05-29 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant gene silencing is a crucially important phenomenon in gene expression and epigenetics. This book describes the way small RNA is produced and acts to silence genes, its likely origins in defence against viruses, and also its potential to improve plants. Plant gene silencing can be used to improve industrial traits, make plants more nutritious or more valuable to consumers, to remove allergens, and to improve resistance to weeds and pathogens.

Author : Renato Paro
Publisher : Springer Nature
ISBN 13 : 3030686701
Total Pages : 215 pages
Book Rating : 4.03/5 ( download)

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Download or read book Introduction to Epigenetics written by Renato Paro and published by Springer Nature. This book was released on 2021-03-23 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access textbook leads the reader from basic concepts of chromatin structure and function and RNA mechanisms to the understanding of epigenetics, imprinting, regeneration and reprogramming. The textbook treats epigenetic phenomena in animals, as well as plants. Written by four internationally known experts and senior lecturers in this field, it provides a valuable tool for Master- and PhD- students who need to comprehend the principles of epigenetics, or wish to gain a deeper knowledge in this field. After reading this book, the student will: Have an understanding of the basic toolbox of epigenetic regulation Know how genetic and epigenetic information layers are interconnected Be able to explain complex epigenetic phenomena by understanding the structures and principles of the underlying molecular mechanisms Understand how misregulated epigenetic mechanisms can lead to disease

Author : Grace W. Redberry
Publisher : Nova Publishers
ISBN 13 : 9781594548321
Total Pages : 216 pages
Book Rating : 4.23/5 ( download)

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Download or read book Gene Silencing written by Grace W. Redberry and published by Nova Publishers. This book was released on 2006 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene silencing is a general term describing epigenetic processes of gene regulation. The term gene silencing is generally used to describe the 'switching off' of a gene by a mechanism other than genetic mutation. That is, a gene which would be expressed (turned on) under normal circumstances, is switched off by machinery in the cell. Genes are regulated at either the transcriptional or post-transcriptional level. Transcriptional gene silencing is the result of histone modifications, creating an environment of heterochromatin around a gene that makes it inaccessible to transcriptional machinery (RNA polymerase, transcription factors, etc.). Post-transcriptional gene silencing is the result of mRNA of a particular gene being destroyed. The destruction of the mRNA prevents translation to form an active gene product (in most cases, a protein). A common mechanism of post-transcriptional gene silencing is RNAi. Both transcriptional and post-transcriptional gene silencing are used to regulate endogenous genes. This book presents the latest research in this important field.

Author : Esra Galun
Publisher : World Scientific
ISBN 13 : 9812562060
Total Pages : 469 pages
Book Rating : 4.67/5 ( download)

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Download or read book RNA Silencing written by Esra Galun and published by World Scientific. This book was released on 2005 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on an emerging, central issue in molecular genetics and the development of eukaryotes: the control of gene expression by small species of RNA. As an exciting new field of endeavor, it is the first book by a single author to deal comprehensively with RNA silencing.The book provides the historical background of the field preceding the seminal work by Fire and associates in 1998 on the impact of small double-stranded RNA on the expression of nematode genes, which is considered the beginning of RNA silencing research. RNA silencing is described in a wide range of plants and animals including protozoa, simple metazoa, insects, non-mammalian vertebrates, and mammals. In each case the experimental results are provided with the accompanying background and with illustrations. There is also an appendix on the prospective use of RNA silencing in gene therapy, which is intended as a guide for investigators wishing to explore this possibility.

Author : Shiv Sanjeevi Sripathi
Publisher : Delve Publishing
ISBN 13 : 9781774076965
Total Pages : pages
Book Rating : 4.69/5 ( download)

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Download or read book Gene Silencing: Approaches, Applications and Updates written by Shiv Sanjeevi Sripathi and published by Delve Publishing. This book was released on 2020-11 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: We turn off switches every day-can we turn off a gene or a group of genes? Sounds exciting? Read ahead. Researchers are using the property of RNA interference to silence several genes that have opened up the use of siRNAs in the analysis of the various genes that are involved or implicated in diseases. This book is a concise presentation of RNA silencing: the mechanisms involved have been discussed along with figures. The various patents, research strategies and the market for RNAi-based therapeutics have also been presented for the readers to understand the exact scenario in this one concise book that will "silence" your questions!

Author : Kirankumar S. Mysore
Publisher : Humana
ISBN 13 : 9781493924523
Total Pages : 0 pages
Book Rating : 4.24/5 ( download)

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Download or read book Plant Gene Silencing written by Kirankumar S. Mysore and published by Humana. This book was released on 2015-03-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.