Enzymes Mechanisms Dynamics And Inhibition

Author :
Publisher : Academic Press
ISBN 13 : 0128177632
Total Pages : 332 pages
Book Rating : 4.31/5 ( download)

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Download or read book Enzymes – Mechanisms, Dynamics and Inhibition written by and published by Academic Press. This book was released on 2020-09-21 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes – Mechanisms, Dynamics and Inhibition, Volume 122, the latest release in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting new and interesting chapters on the topics. Each chapter is written by an international board of authors. Provides a targeted approach to a very wide audience of specialists, researchers and students Contains timely chapters written by well-renowned authorities in their field Includes a number of high quality illustrations, figures and tables

Author : Michael I. Page
Publisher :
ISBN 13 :
Total Pages : 576 pages
Book Rating : 4.92/5 ( download)

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Download or read book Enzyme Mechanisms written by Michael I. Page and published by . This book was released on 1987 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Theories of enzyme catalysis. Enzyme models - synthetic polymers. Enzyme models - crown ethers. Enzyme models - cyclodextrins (cycloamyloses). Enzyme models - small molecule and intramolecular catalysis. Use of protein engineering to study enzyme mechanisms. Transition state affinity and the design of enzyme inhibitors. Acyl group transfer -fundamental mechanisms, cysteine proteinases, the serine proteinases, phosphoryl transfer, sulphotransferases and sulphatases, aspartic proteinases, metalloproteinases. Glycosil group transfer. Isomerization mechanisms through hydrogem and carbon transfer. Imine formation in enzimatic reactions. Pyridoxal phosphate dependent enzymes. Thiamine-depedent enzymes. Adenosylcobalamin-dependent enzymic reactions. Folate-dependent enzymes. Glutathione-dependent enzymes-chemistry. Glutathione-dependent enzymes - glutathione-S-transferases. Oxido-reductases - pyridine nucleotide-dependent enzymes. Oxido-reductases - flavoenzymes. Multi-enzyme complexes - Eukaryotic fatty acid synthases.

Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
ISBN 13 : 0306480255
Total Pages : 237 pages
Book Rating : 4.56/5 ( download)

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Download or read book Enzyme Kinetics and Mechanisms written by Kenneth B. Taylor and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.

Author : Antonio Baici
Publisher : Springer
ISBN 13 : 3709114020
Total Pages : 503 pages
Book Rating : 4.25/5 ( download)

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Download or read book Kinetics of Enzyme-Modifier Interactions written by Antonio Baici and published by Springer. This book was released on 2015-06-24 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.

Author :
Publisher : Academic Press
ISBN 13 : 0123847443
Total Pages : 703 pages
Book Rating : 4.47/5 ( download)

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Download or read book Contemporary Enzyme Kinetics and Mechanism written by and published by Academic Press. This book was released on 2009-10-24 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Author : Murat Şentürk
Publisher : BoD – Books on Demand
ISBN 13 : 9535130579
Total Pages : 270 pages
Book Rating : 4.74/5 ( download)

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Download or read book Enzyme Inhibitors and Activators written by Murat Şentürk and published by BoD – Books on Demand. This book was released on 2017-03-29 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the recent years, medicinal chemistry has become responsible for explaining interactions of chemical molecule processes such that many scientists in the life sciences from agronomy to medicine are engaged in medicinal research. This book contains an overview focusing on the research area of enzyme inhibitor and activator, enzyme-catalyzed biotransformation, usage of microbial enzymes, enzymes associated with programmed cell death, natural products as potential enzyme inhibitors, protease inhibitors from plants in insect pest management, peptidases, and renin-angiotensin system. The book provides an overview on basic issues and some of the recent developments in medicinal science and technology. Especially, emphasis is devoted to both experimental and theoretical aspect of modern medicine. The primary target audience for the book includes students, researchers, chemists, molecular biologists, medical doctors, pharmacologists, and professionals who are interested in associated areas. The textbook is written by international scientists with expertise in biochemistry, enzymology, molecular biology, and genetics, many of which are active in biochemical and pharmacological research. I would like to acknowledge the authors for their contribution to the book. We hope that the textbook will enhance the knowledge of scientists in the complexities of some medical approaches; it will stimulate both professionals and students to dedicate part of their future research in understanding relevant mechanisms and applications of pharmacology.

Author : Robert A. Copeland
Publisher : John Wiley & Sons
ISBN 13 : 0471461857
Total Pages : 416 pages
Book Rating : 4.52/5 ( download)

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Download or read book Enzymes written by Robert A. Copeland and published by John Wiley & Sons. This book was released on 2004-04-07 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research.

Author : Daniel L. Purich
Publisher : Academic Press
ISBN 13 : 1483214222
Total Pages : 567 pages
Book Rating : 4.21/5 ( download)

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Download or read book Contemporary Enzyme Kinetics and Mechanism written by Daniel L. Purich and published by Academic Press. This book was released on 1983-01-01 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Author : Rakesh Sharma
Publisher : BoD – Books on Demand
ISBN 13 : 953510585X
Total Pages : 334 pages
Book Rating : 4.55/5 ( download)

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Download or read book Enzyme Inhibition and Bioapplications written by Rakesh Sharma and published by BoD – Books on Demand. This book was released on 2012-05-09 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Inhibition and Bioapplications is a concise book on applied methods of enzymes used in drug testing. The present volume will serve the purpose of applied drug evaluation methods in research projects, as well as relatively experienced enzyme scientists who might wish to develop their experiments further. Chapters are arranged in the order of basic concepts of enzyme inhibition and physiological basis of cytochromes followed by new concepts of applied drug therapy; reliability analysis; and new enzyme applications from mechanistic point of view.

Author : Paul F. Cook
Publisher : Garland Science
ISBN 13 : 1136844287
Total Pages : 416 pages
Book Rating : 4.87/5 ( download)

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Download or read book Enzyme Kinetics and Mechanism written by Paul F. Cook and published by Garland Science. This book was released on 2007-03-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.