Electron Microscopy In Microbiology

Author : Michael J. Dykstra
Publisher : Springer Science & Business Media
ISBN 13 : 146840010X
Total Pages : 368 pages
Book Rating : 4.06/5 ( download)

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Download or read book Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

Author : Ray Egerton
Publisher : Springer Science & Business Media
ISBN 13 : 9780387258003
Total Pages : 224 pages
Book Rating : 4.00/5 ( download)

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Download or read book Physical Principles of Electron Microscopy written by Ray Egerton and published by Springer Science & Business Media. This book was released on 2011-02-11 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning and stationary-beam electron microscopes are indispensable tools for both research and routine evaluation in materials science, the semiconductor industry, nanotechnology and the biological, forensic, and medical sciences. This book introduces current theory and practice of electron microscopy, primarily for undergraduates who need to understand how the principles of physics apply in an area of technology that has contributed greatly to our understanding of life processes and "inner space." Physical Principles of Electron Microscopy will appeal to technologists who use electron microscopes and to graduate students, university teachers and researchers who need a concise reference on the basic principles of microscopy.

Author : John J. Bozzola
Publisher : Jones & Bartlett Learning
ISBN 13 : 9780763701925
Total Pages : 702 pages
Book Rating : 4.20/5 ( download)

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Download or read book Electron Microscopy written by John J. Bozzola and published by Jones & Bartlett Learning. This book was released on 1999 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

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Publisher : Academic Press
ISBN 13 : 9780080860497
Total Pages : 431 pages
Book Rating : 4.94/5 ( download)

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Download or read book Electron Microscopy in Microbiology written by and published by Academic Press. This book was released on 1988-10-01 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and macromolecular levels. Many of the recent findings on ultrastructural features of microorganisms have been obtained with newly developed methods, though classical approaches have not lost their validity. Therefore, both conventional and new methods have been incorporated into this volume. The topics dealt with are meaningful not only in bacterial cytology but also in physiology, enzymology, biochemistry, and molecular biology, and include aspects of medical and biotechnological application.

Author : J.K. Koehler
Publisher : Springer Science & Business Media
ISBN 13 : 3642654924
Total Pages : 319 pages
Book Rating : 4.23/5 ( download)

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Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Author : Joseph Goldstein
Publisher : Springer Science & Business Media
ISBN 13 : 1461332737
Total Pages : 679 pages
Book Rating : 4.32/5 ( download)

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Download or read book Scanning Electron Microscopy and X-Ray Microanalysis written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.

Author : James Pawley
Publisher : Springer Science & Business Media
ISBN 13 : 0387729720
Total Pages : 323 pages
Book Rating : 4.25/5 ( download)

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Download or read book Biological Low-Voltage Scanning Electron Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2007-12-03 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Author : M. A. Nasser Hajibagheri
Publisher : Springer Science & Business Media
ISBN 13 : 1592592015
Total Pages : 292 pages
Book Rating : 4.12/5 ( download)

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Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Author : R.G. Kessel
Publisher : Springer Science & Business Media
ISBN 13 : 3642808344
Total Pages : 330 pages
Book Rating : 4.40/5 ( download)

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Download or read book Scanning Electron Microscopy in BIOLOGY written by R.G. Kessel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the continuing quest to explore structure and to relate struc tural organization to functional significance, the scientist has developed a vast array of microscopes. The scanning electron microscope (SEM) represents a recent and important advance in the development of useful tools for investigating the structural organization of matter. Recent progress in both technology and methodology has resulted in numerous biological publications in which the SEM has been utilized exclusively or in connection with other types of microscopes to reveal surface as well as intracellular details in plant and animal tissues and organs. Because of the resolution and depth of focus presented in the SEM photograph when compared, for example, with that in the light microscope photographs, images recorded with the SEM have widely circulated in newspapers, periodicals and scientific journals in recent times. Considering the utility and present status of scanning electron microscopy, it seemed to us to be a particularly appropriate time to assemble a text-atlas dealing with biological applications of scanning electron microscopy so that such information might be presented to the student and to others not yet familiar with its capabilities in teaching and research. The major goal of this book, therefore, has been to assemble material that would be useful to those students beginning their study of botany or zoo logy, as well as to beginning medical students and students in advanced biology courses.

Author : Thomas Muller-Reichert
Publisher : Academic Press
ISBN 13 : 0124160263
Total Pages : 466 pages
Book Rating : 4.62/5 ( download)

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Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods