Dynamic Force Spectroscopy And Biomolecular Recognition

Author : Anna Rita Bizzarri
Publisher : CRC Press
ISBN 13 : 1439862389
Total Pages : 278 pages
Book Rating : 4.84/5 ( download)

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Download or read book Dynamic Force Spectroscopy and Biomolecular Recognition written by Anna Rita Bizzarri and published by CRC Press. This book was released on 2012-01-25 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies a

Author : Anna Rita Bizzarri
Publisher : CRC Press
ISBN 13 : 1439862370
Total Pages : 288 pages
Book Rating : 4.77/5 ( download)

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Download or read book Dynamic Force Spectroscopy and Biomolecular Recognition written by Anna Rita Bizzarri and published by CRC Press. This book was released on 2012-01-25 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies and is finding wider applications in areas such as biophysics and polymer science. In six chapters, Dynamic Force Spectroscopy and Biomolecular Recognition covers the most recent ideas and advances in the field of DFS applied to biorecognition: Chapter 1: Reviews the basic and novel aspects of biorecognition and discusses the emerging capabilities of single-molecule techniques to disclose kinetic properties and molecular mechanisms usually hidden in bulk measurements Chapter 2: Describes the basic principle of atomic force microsocopy (AFM) and DFS, with particular attention to instrumental and theoretical aspects more strictly related to the study of biomolecules Chapter 3: Overviews the theoretical background in which experimental data taken in nonequilibrum measurements of biomolecular unbinding forces are extrapolated to equilibrium conditions Chapter 4: Reviews the most common and efficient strategies adopted in DFS experiments to immobilize the interacting biomolecules to the AFM tip and to the substrate Chapter 5: Presents and discusses the most representative aspects related to the analysis of DFS data and the challenges of integrating well-defined criteria to calibrate data in automatic routinary procedures Chapter 6: Overviews the most relevant DFS applications to study biorecognition processes, including the biotin/avidin pair, and selected results on various biological complexes, including antigen/antibody, proteins/DNA, and complexes involved in adhesion processes Chapter 7: Summarizes the main results obtained by DFS applied to study biorecognition processes with forthcoming theoretical and experimental advances Although DFS is a widespread, worldwide technique, no books focused on this subject have been available until now. Dynamic Force Spectroscopy and Biomolecular Recognition provides the state of the art of experimental data analysis and theoretical procedures, making it a useful tool for researchers applying DFS to study biorecognition processes.

Author : Jingfeng Li
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.03/5 ( download)

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Download or read book Measuring Molecular Recognition Between Biomolecules with AFM-based Single-molecule Force Spectroscopy for Label-free Biosensing written by Jingfeng Li and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Aleksandr Noy
Publisher : Springer Science & Business Media
ISBN 13 : 038749989X
Total Pages : 311 pages
Book Rating : 4.95/5 ( download)

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Download or read book Handbook of Molecular Force Spectroscopy written by Aleksandr Noy and published by Springer Science & Business Media. This book was released on 2007-12-03 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: Researchers in academia and industry who are interested in techniques for measuring intermolecular forces will find this an essential text. It presents a review of modern force spectroscopy, including fundamentals of intermolecular forces, technical aspects of the force measurements, and practical applications. The handbook begins with a review of the fundamental physics of loading single and multiple chemical bonds on the nanometer scale. It contains a discussion of thermodynamic and kinetic models of binding forces and dissipation effects in nanoscale molecular contacts, covers practical aspects of modern single-molecule level techniques, and concludes with applications of force spectroscopy to chemical and biological processes. Computer modeling of force spectroscopy experiments is also addressed.

Author : Malgorzata Lekka
Publisher : CRC Press
ISBN 13 : 1315341158
Total Pages : 217 pages
Book Rating : 4.56/5 ( download)

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Download or read book Cellular Analysis by Atomic Force Microscopy written by Malgorzata Lekka and published by CRC Press. This book was released on 2017-07-06 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite substantial evidence showing the feasibility of Atomic Force Microscopy (AFM) to identify cells with altered elastic and adhesive properties, the use of this technique as a complementary diagnostic method remains controversial. This book is designed to be a practical textbook that teaches how to assess the mechanical characteristics of living, individual cells by AFM. Following a step-by-step approach, it introduces the methodology of measurements in the case of both determination of elastic properties and quantification of adhesive properties.

Author :
Publisher : Walter de Gruyter
ISBN 13 : 3110298341
Total Pages : 452 pages
Book Rating : 4.45/5 ( download)

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Download or read book Complex Flavoproteins, Dehydrogenases and Physical Methods written by and published by Walter de Gruyter. This book was released on 2013-08-01 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: The dynamic field of flavin and flavoprotein biochemistry has seen rapid advancement in recent years. This comprehensive two volume set provides an overview of all aspects of contemporary research in this important class of enzymes. Topics treated include flavoproteins involved in energy generation, signal transduction and electron transfer (including respiration); oxygen activation by flavoproteins; the biology and biochemistry of complex flavoproteins; flavin and flavoprotein photochemistry/photophysics as well as biotechnological applications of flavoproteins. Recent developments in this field include new structures (including those of large membrane-integral electron transfer complexes containing FMN or FAD), elucidation of the role of flavoproteins in cell signalling pathways (including both phototaxis and the circadian cycle) and important new insights into the reaction mechanisms of flavin-containing enzymes. This volume focusing on complex flavoproteins and physical methods is an essential reference for all researchers in biochemistry, chemistry, photochemistry and photophysics working on flavoenzymes.

Author : Carolin Madwar
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.85/5 ( download)

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Download or read book Studying Biomolecular Interactions Using Atomic Force Microscopy written by Carolin Madwar and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many biological systems involve mechanical interactions and specific molecular recognition events that are essential for their function. Single molecule force spectroscopy (SMFS) is a powerful and versatile atomic force microscopy (AFM)-based technique, which allows probing such interactions at the single molecule level, with piconewton sensitivity, thereby illuminating their dynamics as well as their structural and mechanical properties. A fundamental requirement in these studies is the immobilization of biomolecules between the AFM probe and the sample surface, preferably by a long flexible molecular spacer, such as poly(ethylene glycol) (PEG). The goal of this project is to investigate the binding interactions in four distinct biomolecular systems at the single molecule level, using SMFS. A new amination strategy was used to attach a novel bifunctional PEG spacer containing amine- and thiol-reactive termini. Biomolecules under investigation were tethered to AFM tips by formation of covalent linkages to this versatile spacer. Various tests, including surface plasmon resonance and a UV-based enzyme assay, were carried out to evaluate and confirm AFM tip functionalization. SMFS of biotin/streptavidin yielded an average unbinding force of 59 pN at 4000 pN/s loading rate. SMFS of concanavalin A/mannose yielded an average unbinding force of 50 pN at loading rate of 6000 pN/s. SMFS of a de novo heterodimeric E/K coiled/coil yielded an average unbinding force of 41 pN at 7000 pN/s loading rate with a k off rate calculated to be 15.8 s-1 . SMFS of these biomolecular systems were not only successful and informative, but also provide exciting directions towards future applications.

Author : Xiaojuan Zhang
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.89/5 ( download)

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Download or read book Investigation of Biomolecular Interactions for Development of Sensors and Diagnostics written by Xiaojuan Zhang and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The highly specific recognition processes between biomolecules mediate various crucial biological processes. Uncovering the molecular basis of these interactions is of great fundamental and applied importance. This research work focuses on understanding the interactions of several biomolecular recognition systems and processes that can provide fundamental information to aid in the rational design of sensing and molecular recognition tools. Initially, a reliable and versatile platform was developed to investigate biomolecular interactions at a molecular level. This involved several techniques, including biomolecule functionalization to enable attachment to self-assembled monolayers as well as atomic force microscopy (AFM) based force spectroscopy to uncover the binding or rupture forces between the receptor and ligand pairs. It was shown that this platform allowed determination of molecular binding between single molecules with a high specificity. The platform was further adapted to a general sensing formulation utilizing a group of flexible and adaptive nucleic acid recognition elements (RNA and DNA aptamers) to detect specific target proteins. Investigation of interactions at the molecular level allowed characterization of the dynamics, specificity and the conformational properties of these functional nucleic acids in a manner inaccessible via traditional interaction studies. These interactions were then adapted to aptamer-based detecting methods that at the ensemble or bulk scale, specifically taking advantage of mechanisms uncovered in the biophysical study of this system. A quartz crystal microbalance (QCM) was used to detect protein targets at the bulk level and the affinities and binding kinetics of these systems were analyzed. Along with AFM-based force spectroscopy, ensemble-averaging properties and molecular properties of these interactions could be correlated to contribute to bridging the gap across length scales. Finally, more broadly applicable sensing platform was developed to take advantage of the unique properties of aptamers. DNA was employed both as a carrier and as a molecular recognition agent. DNA was used as a template for nanoconstruction and fabricating unique shapes that could enhance the aptamer-based molecular recognition strategies. With aptamers tagged to distinct nanoconstructed DNA, a novel shape-based detecting method was enabled at the molecular level. The results demonstrated that this is a flexible strategy, which can be further developed as ultrasensitive single molecule sensing strategy in complex environments.

Author : Nuno C. Santos
Publisher : Humana
ISBN 13 : 9781493994076
Total Pages : 372 pages
Book Rating : 4.77/5 ( download)

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Download or read book Atomic Force Microscopy written by Nuno C. Santos and published by Humana. This book was released on 2019-12-10 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

Author : P. Carmona
Publisher : Springer Science & Business Media
ISBN 13 : 9401156220
Total Pages : 661 pages
Book Rating : 4.26/5 ( download)

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Download or read book Spectroscopy of Biological Molecules: Modern Trends written by P. Carmona and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 661 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 1997 European Conference on Spectroscopy of Biological Molecules (ECSBM) is the seventh in a biennial series of conferences devoted to the applications of molecular spectroscopy to biological molecules and related systems. The interest of these conferences rests mainly on the relationship between the structure and physiological activity of biological molecules and related systems of which these molecular species form part. This volume ofECSBM contains articles prepared by the invited lecturers and those making poster presentations at the seventh ECSBM. The reader will find mainly applications of vibrational spectroscopy to protein structure and dynamics, biomembranes, molecular recognition, nucleic acids and other biomolecules and biological systems containing specific chromophors. Biomedical applications of vibrational spectroscopy are expanding rapidly. On the other hand, a significant number of the papers describe applications of other methods, such as NMR, circular dichroism, optical absorption and fluorescence, X-ray absorption and diffraction and other theoretical methods. One aim has been to achieve a well balanced, critically comparative review of recent progress in the field of biomolecular structure, bonding and dynamics based on applications of the above spectroscopic methods. A great part of the contributions included in this volume are devoted to biomedical and biotechnological applications and provide a broadly based account of recent applicationS in this field. The content of this book has been organized in sections corresponding mainly to the different types of biological molecules investigated. This book includes also another section related to theoretical methods where MO calculations of vibrational frequencies dominate clearly the topic.