Drosophila Cytogenetics Protocols

Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
ISBN 13 : 1592596657
Total Pages : 467 pages
Book Rating : 4.52/5 ( download)

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Download or read book Drosophila Cytogenetics Protocols written by Daryl S. Henderson and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Author : William Sullivan
Publisher : CSHL Press
ISBN 13 : 9780879695866
Total Pages : 798 pages
Book Rating : 4.62/5 ( download)

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Download or read book Drosophila Protocols written by William Sullivan and published by CSHL Press. This book was released on 2000 with total page 798 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Author : Paul Popescu
Publisher : Springer Science & Business Media
ISBN 13 : 9783540667377
Total Pages : 260 pages
Book Rating : 4.77/5 ( download)

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Download or read book Techniques in Animal Cytogenetics written by Paul Popescu and published by Springer Science & Business Media. This book was released on 2000-08-15 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The theoretical background of cytogenetics as well as the practical methods to investigate chromosomes from Drosophila to mammals and humans are presented in this book. Theory and practical protocols on the following topics are included: Cell culture methods, chromosome preparation and banding techniques, radioactive and fluorescent in situ hybridisation (FISH) methods and their derivates (chromosome painting), meiotic chromosome methods using light and electronic microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes as well as flow and slit scan cytometry. The editors, all at INRA, played a prominent part in the development of cytogenetics. Paul Popescu, a specialist for domestic animals worked with gene mapping, Helene Hayes, together with Popescu showed the homology of bovine and human chromosome disorders, and Bernard Dutrilaux focused on human cytogenetics.

Author : Daryl S. Henderson
Publisher : Humana Press
ISBN 13 : 9781588290502
Total Pages : 468 pages
Book Rating : 4.06/5 ( download)

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Download or read book Drosophila Cytogenetics Protocols written by Daryl S. Henderson and published by Humana Press. This book was released on 2003-11-04 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Author : Christian Dahmann
Publisher : Humana Press
ISBN 13 : 9781617377914
Total Pages : 438 pages
Book Rating : 4.10/5 ( download)

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Download or read book Drosophila written by Christian Dahmann and published by Humana Press. This book was released on 2010-12-10 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drosophila is a comprehensive collection of methods and protocols for Drosophila, one of the oldest and most commonly used model organisms in modern biology. The protocols are written by the scientists who invented the methods. The text presents a diverse set of techniques that range from the basic handling of flies to more complex applications. This is the perfect reference manual for Drosophila researchers.

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.02/5 ( download)

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Download or read book Molecular Cytogenetics written by and published by . This book was released on 1973 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Hank Bass
Publisher : Springer Science & Business Media
ISBN 13 : 0387708693
Total Pages : 346 pages
Book Rating : 4.90/5 ( download)

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Download or read book Plant Cytogenetics written by Hank Bass and published by Springer Science & Business Media. This book was released on 2011-12-02 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book provides information on plant cytogenetics for students, instructors, and researchers. Topics covered by international experts include classical cytogenetics of plant genomes; plant chromosome structure; functional, molecular cytology; and genome dynamics. In addition, chapters are included on several methods in plant cytogenetics, informatics, and even laboratory exercises for aspiring or practiced instructors. The book provides a unique combination of historical and modern subject matter, revealing the central role of plant cytogenetics in plant genetics and genomics as currently practiced. This breadth of coverage, together with the inclusion of methods and instruction, is intended to convey a deep and useful appreciation for plant cytogenetics. We hope it will inform and inspire students, researchers, and teachers to continue to employ plant cytogenetics to address fundamental questions about the cytology of plant chromosomes and genomes for years to come. Hank W. Bass is a Professor in the Department of Biological Science at Florida State University. James A. Birchler is a Professor in the Division of Biological Sciences at the University of Missouri.

Author : Sumitra Deb
Publisher : Springer Science & Business Media
ISBN 13 : 1592594085
Total Pages : 282 pages
Book Rating : 4.85/5 ( download)

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Download or read book p53 Protocols written by Sumitra Deb and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein. Several unique properties of p53 posed a challenge to understa- ing its normal function in the initial phase of its research. The low levels of p53 in normal cells, its stabilization under situations of genotoxic stress, induction of growth arrest, and apoptosis with stabilization of the protein, obstructed the visibility of its normal, unmutated function. The property of p53 that can sense a promoter and transactivate or inhibit is still not well understood. It is still not known whether it is the absence of the protein that causes tumorigenesis, or if its mutants have a dominant role in inducing cancer. p53 Protocols comprises eighteen chapters for the study of the diverse properties of p53 and related proteins. The methods included are invaluable for delineating the function of other proteins that may function as tumor suppr- sors or growth suppressors. The chapters are not presented in any schematic order, for the importance and diversity of the functions of p53 make it imp- sible to organize them suitably. We have made a sincere effort to collect the methods most useful to those investigators working on tumor suppressors or growth suppressors. The purpose of p53 Protocols is not only to provide investigators with methods to analyze similar biochemical functions, but also to familiarize them with the associated problems that arose during the course of investigations.

Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
ISBN 13 : 159259400X
Total Pages : 330 pages
Book Rating : 4.09/5 ( download)

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Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Author : John M. S. Bartlett
Publisher : Springer Science & Business Media
ISBN 13 : 1592593844
Total Pages : 1083 pages
Book Rating : 4.42/5 ( download)

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Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.