Dna Cloning A Hands On Approach

Author : Seok-Yong Choi
Publisher : Springer
ISBN 13 : 9402416625
Total Pages : 131 pages
Book Rating : 4.26/5 ( download)

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Download or read book DNA Cloning: A Hands-on Approach written by Seok-Yong Choi and published by Springer. This book was released on 2019-04-17 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Author : Seok-Yong Choi
Publisher :
ISBN 13 : 9789402416619
Total Pages : 139 pages
Book Rating : 4.17/5 ( download)

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Download or read book DNA Cloning written by Seok-Yong Choi and published by . This book was released on 2019 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Author : David M. Glover
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.11/5 ( download)

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Download or read book DNA Cloning written by David M. Glover and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : David M. Glover
Publisher :
ISBN 13 :
Total Pages : 269 pages
Book Rating : 4.85/5 ( download)

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Download or read book DNA Cloning written by David M. Glover and published by . This book was released on 1995 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : David M. Glover
Publisher :
ISBN 13 :
Total Pages : 269 pages
Book Rating : 4.69/5 ( download)

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Download or read book DNA Cloning written by David M. Glover and published by . This book was released on 1995 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : David M. Glover
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.02/5 ( download)

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Download or read book DNA cloning written by David M. Glover and published by . This book was released on 1986 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : D. M. Glover
Publisher :
ISBN 13 : 9780947946197
Total Pages : 245 pages
Book Rating : 4.95/5 ( download)

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Download or read book DNA Cloning written by D. M. Glover and published by . This book was released on 1985 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Joseph Sambrook
Publisher :
ISBN 13 : 9780879695774
Total Pages : 0 pages
Book Rating : 4.73/5 ( download)

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Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Heather B. Miller
Publisher : Academic Press
ISBN 13 : 0123855454
Total Pages : 227 pages
Book Rating : 4.59/5 ( download)

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Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author :
Publisher : Elsevier
ISBN 13 : 0124199542
Total Pages : 405 pages
Book Rating : 4.45/5 ( download)

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Download or read book Laboratory Methods in Enzymology: DNA written by and published by Elsevier. This book was released on 2013-09-02 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA