Dna Based Molecular Diagnostic Techniques

Author : Peter Walker
Publisher : Daya Books
ISBN 13 : 9788170354048
Total Pages : 108 pages
Book Rating : 4.48/5 ( download)

DOWNLOAD NOW!

Download or read book DNA Based Molecular Diagnostic Techniques written by Peter Walker and published by Daya Books. This book was released on 2005 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: In efforts to limit transboundary movement of pathogens and reduce the economic and socioeconomic impact of disease in aquaculture, there is considerable scope for more effective use of DNA-based methods of pathogen detection. These technologies offer rapid results with potentially high sensitivity and specificity, at relatively low cost. However, few, if any, of the available tests have been assessed appropriately against other diagnostic methods or standardized and validated for specified applications. Implementation of standardized practices that produce reliable, useful and comparable date will require a significant investment in research, training and infrastructure development. The review recommends the development of programmes of managed cooperative research to assist more effective use of DNA-based detection tests, focusing on improving the knowledge base by identification of new and emerging pathogens, relating pathogens in the Asia region to those described elsewhere and defining the extent of genetic variation between related pathogens. The review also recommends the development of a laboratory accreditation programme in order to achieve standardization of sampling methods and test procedures complementary to the activities of the International Office of Epizootics (OIE) in obtaining internationally agreed test standards for molecular diagnostic technologies. Contents Chapter 1: Background; Global aquaculture development, Impact of disease, Role of DNA-based technologies in diagnosis and pathogen detection, Impediments to the use of DNA-based diagnostic technique; Chapter 2: Expert Workshop; Terms of reference, Participants, Workshop process; Chapter 3: Pathogen Focus Group Reports; Shrimp pathogens, Status of research and identification of research needs, Standardization of DNA-based diagnostic tests, Networking and communication, Training and extension, Mollusc pathogens, Finfish pathogens, Diseases requiring applicaition of DNA-based technologies, Evaluation of needs for rapid detection techniques, Status of research, Key constraints to establishment of international standards, Recommendations for research program; Chapter 4: Issue Relating to the Adoption of International Standards for Disease Diagnosis and Health Certification; Chapter 5: Key Outcomes and Recommendations; General comments, Research needs, Training needs, Communication needs, International standardisation; Chapter 6: References; Chapter 7: Contributed Papers and Reviews; Technological constraints to disease prevention and control in aquatic animals, with special reference to pathogen detection by Sharon MC Gladdery, The absolute requirement for predictive validation of non-culture based detection techniques by Maura Hiney, Evaluation of diagnostic tests: the epidemiological approach by Daniel F Fegan, DNA-based diagnostic and detection methods for penaeid shrimp viral diseases by Donald V Lightner, Practical problems with PCR detection in Asia: the importance of standardization by M Shariff, S Soon, K L Lee and L T Tan, Application of polymerase chain reaction for detection of shrimp pathogens in India by Indrani Karunasagar, Viral genetic variation: implications for disease diagnosis and detection of shrimp pathogens by Peter J Walker and Jeff A Cowley, The need for molecular tools in the study of mollusc pathogens by P m Hine, Development and validation of DNA-based diagnostic techniques with particular reference to bivalve mollusc pathogens by Franck C J Berthe, Molecular diagnostics for the oyster pathogens haplosporidium nelsoni (Msx disease) and perkinsus marinus (Dermo disease) in chesapeake bay, Virginia, USA by Eugene M Burreson, Rapid detection and identification of fish pathogens by Alexandra Adams, DNA-based studies on aphanomyces invadans, the fungal pathogen of epizootic ulcerative syndrome (Eus) by James H Lilley and Supranee Chanbut, Importance of standardisation and validation of molecular diagnostic techniques for aquatic animal pathogens and diseases for international trade and trans-boundary movement of aquatic animals and animal products by Barry J Hill.

Author : Food and Agriculture Organization of the United Nations
Publisher : Food & Agriculture Org.
ISBN 13 : 9789251044209
Total Pages : 104 pages
Book Rating : 4.01/5 ( download)

DOWNLOAD NOW!

Download or read book DNA-based Molecular Diagnostic Techniques written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2000 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the report , including recommendations, and 13 papers presented, of the Expert Workshop held in Bangkok, Thailand, from 7-9 February 1999. The workshop found that there is considerable scope for more effective use of DNA-based methods of pathogen detection to limit transboundary movement of pathogens & reduce the impact of disease in aquaculture. Few if any, of the available tests have been assessed appropriately or standardized and validated. It is recommended that programmes are developed to manage cooperative research to assist more effective use of DNA-based detection tests and that a laboratory accreditation programmed to achieve standardization also be developed

Author : George P. Patrinos
Publisher : Academic Press
ISBN 13 : 0128029889
Total Pages : 520 pages
Book Rating : 4.86/5 ( download)

DOWNLOAD NOW!

Download or read book Molecular Diagnostics written by George P. Patrinos and published by Academic Press. This book was released on 2016-10-27 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field

Author : Nader Rifai
Publisher : Elsevier
ISBN 13 : 0128160624
Total Pages : 422 pages
Book Rating : 4.26/5 ( download)

DOWNLOAD NOW!

Download or read book Principles and Applications of Molecular Diagnostics written by Nader Rifai and published by Elsevier. This book was released on 2018-06-13 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. Explains the principles and tools of molecular biology Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results Provides a detailed description of current molecular applications used to solve diagnostics tasks

Author : Varsha Gupta
Publisher : Springer
ISBN 13 : 9811008752
Total Pages : 527 pages
Book Rating : 4.57/5 ( download)

DOWNLOAD NOW!

Download or read book Basic and Applied Aspects of Biotechnology written by Varsha Gupta and published by Springer. This book was released on 2016-10-22 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.

Author : National Academies of Sciences, Engineering, and Medicine
Publisher : National Academies Press
ISBN 13 : 0309486564
Total Pages : 121 pages
Book Rating : 4.69/5 ( download)

DOWNLOAD NOW!

Download or read book Developing Norms for the Provision of Biological Laboratories in Low-Resource Contexts written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2019-05-01 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: On June 27-28, 2018, the U.S. National Academies of Sciences, Engineering, and Medicine (the National Academies) convened an international workshop in Amsterdam, the Netherlands, on developing norms for the provision of laboratories in low-resource contexts. The U.S. Department of State's Biosecurity Engagement Program requested that the National Academies organize this workshop to engage an international group of organizations that provide funding for construction, upgrades, and maintenance of biological laboratories in countries without the means to build such labs themselves. Twenty-one people from 19 organizations participated. The intent was to advance the conversation about the identification and application of guiding principles and common norms for use by these organizations in their grants, partnerships, and aid. This publication summarizes the presentations and discussions from the workshop.

Author : Chang-Hui Shen
Publisher : Elsevier
ISBN 13 : 0323986099
Total Pages : 590 pages
Book Rating : 4.90/5 ( download)

DOWNLOAD NOW!

Download or read book Diagnostic Molecular Biology written by Chang-Hui Shen and published by Elsevier. This book was released on 2023-06-29 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)

Author : Wayne W. Grody
Publisher : Academic Press
ISBN 13 : 9780080919041
Total Pages : 736 pages
Book Rating : 4.49/5 ( download)

DOWNLOAD NOW!

Download or read book Molecular Diagnostics written by Wayne W. Grody and published by Academic Press. This book was released on 2009-11-06 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. Molecular Diagnostics provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting. Serves as the definitivereference for molecular pathologists worldwide Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science

Author : William B. Coleman
Publisher : Springer Science & Business Media
ISBN 13 : 1475725884
Total Pages : 387 pages
Book Rating : 4.89/5 ( download)

DOWNLOAD NOW!

Download or read book Molecular Diagnostics written by William B. Coleman and published by Springer Science & Business Media. This book was released on 2013-04-18 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.

Author : E. van Pelt-Verkuil
Publisher : Springer
ISBN 13 : 981131604X
Total Pages : 457 pages
Book Rating : 4.43/5 ( download)

DOWNLOAD NOW!

Download or read book Molecular Diagnostics written by E. van Pelt-Verkuil and published by Springer. This book was released on 2019-06-03 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines. The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith. Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics. Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising: - Clinical microbiology - Clinical chemistry - Clinical genetics - Clinical pathology - Molecular hematopathology - Veterinary health - Plant health - Food safety Both full-colour and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.