Cell Culture In The Neurosciences

Author : Jane Bottenstein
Publisher : Springer Science & Business Media
ISBN 13 : 1461324734
Total Pages : 392 pages
Book Rating : 4.37/5 ( download)

DOWNLOAD NOW!

Download or read book Cell Culture in the Neurosciences written by Jane Bottenstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.

Author : Steven E. Pfeiffer
Publisher : Springer
ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.76/5 ( download)

DOWNLOAD NOW!

Download or read book Neuroscience Approached Thru Cell Culture written by Steven E. Pfeiffer and published by Springer. This book was released on 1982 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Jane Bottenstein
Publisher :
ISBN 13 : 9781461324744
Total Pages : 408 pages
Book Rating : 4.42/5 ( download)

DOWNLOAD NOW!

Download or read book Cell Culture in the Neurosciences written by Jane Bottenstein and published by . This book was released on 1985-05-01 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Laurie C. Doering
Publisher : Humana Press
ISBN 13 : 9781607612919
Total Pages : 400 pages
Book Rating : 4.17/5 ( download)

DOWNLOAD NOW!

Download or read book Protocols for Neural Cell Culture written by Laurie C. Doering and published by Humana Press. This book was released on 2009-10-29 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.

Author : P. Michael Conn
Publisher :
ISBN 13 :
Total Pages : 454 pages
Book Rating : 4.49/5 ( download)

DOWNLOAD NOW!

Download or read book Cell Culture written by P. Michael Conn and published by . This book was released on 1990 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline. Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * Preparation and maintenance of primary and continuous cultures * Cell purification * Bulk isolation * Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures * Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking * Clonal analysis and lineage analysis

Author : S. Fedoroff
Publisher : Elsevier
ISBN 13 : 0323143156
Total Pages : 708 pages
Book Rating : 4.58/5 ( download)

DOWNLOAD NOW!

Download or read book Cell, Tissue, and Organ Cultures in Neurobiology written by S. Fedoroff and published by Elsevier. This book was released on 2012-12-02 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.

Author : Gary Banker
Publisher : MIT Press
ISBN 13 : 9780262024389
Total Pages : 706 pages
Book Rating : 4.81/5 ( download)

DOWNLOAD NOW!

Download or read book Culturing Nerve Cells written by Gary Banker and published by MIT Press. This book was released on 1998 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

Author : Alan A. Boulton
Publisher : Humana Press
ISBN 13 : 9780896033481
Total Pages : 392 pages
Book Rating : 4.81/5 ( download)

DOWNLOAD NOW!

Download or read book Practical Cell Culture Techniques written by Alan A. Boulton and published by Humana Press. This book was released on 1992-09-09 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

Author : George Lees
Publisher :
ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.62/5 ( download)

DOWNLOAD NOW!

Download or read book Cell Culture Approaches to Invertebrate Neuroscience written by George Lees and published by . This book was released on 1988 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Shohreh Amini
Publisher : Humana Press
ISBN 13 : 9781627036412
Total Pages : 165 pages
Book Rating : 4.15/5 ( download)

DOWNLOAD NOW!

Download or read book Neuronal Cell Culture written by Shohreh Amini and published by Humana Press. This book was released on 2013-08-24 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.