Applications Of Hplc In Biochemistry

Author : A. Fallon
Publisher : Elsevier
ISBN 13 : 0080858864
Total Pages : 355 pages
Book Rating : 4.69/5 ( download)

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Download or read book Applications of HPLC in Biochemistry written by A. Fallon and published by Elsevier. This book was released on 1987-07-01 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.

Author : Joel K. Swadesh
Publisher : CRC Press
ISBN 13 : 1420042661
Total Pages : 480 pages
Book Rating : 4.65/5 ( download)

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Download or read book HPLC written by Joel K. Swadesh and published by CRC Press. This book was released on 2000-12-21 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Product specifications, regulatory constraints, and tight production schedules impose considerable pressures on separation scientists in industry. The first edition of HPLC: Practical and Industrial Applications helped eliminate the need for extensive library or laboratory research when confronting a problem, an unfamiliar technique, or work in a n

Author : W.J. Lough
Publisher : CRC Press
ISBN 13 : 9780751400762
Total Pages : 292 pages
Book Rating : 4.69/5 ( download)

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Download or read book High Performance Liquid Chromatography written by W.J. Lough and published by CRC Press. This book was released on 1995-09-30 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Author : S. Pryde
Publisher : Springer Science & Business Media
ISBN 13 : 9780412142208
Total Pages : 288 pages
Book Rating : 4.01/5 ( download)

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Download or read book Applications of High Performance Liquid Chromatography written by S. Pryde and published by Springer Science & Business Media. This book was released on 1979-01-31 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : A. Fallon
Publisher :
ISBN 13 :
Total Pages : 338 pages
Book Rating : 4.11/5 ( download)

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Download or read book Applications of HPLC in Biochemistry written by A. Fallon and published by . This book was released on 1987 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Heinz Engelhardt
Publisher : Springer Science & Business Media
ISBN 13 : 3642692257
Total Pages : 468 pages
Book Rating : 4.53/5 ( download)

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Download or read book Practice of High Performance Liquid Chromatography written by Heinz Engelhardt and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
ISBN 13 : 1592597424
Total Pages : 411 pages
Book Rating : 4.20/5 ( download)

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Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Author : Heinz Engelhardt
Publisher : Springer
ISBN 13 : 9783642692260
Total Pages : 464 pages
Book Rating : 4.65/5 ( download)

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Download or read book Practice of High Performance Liquid Chromatography written by Heinz Engelhardt and published by Springer. This book was released on 2011-12-27 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Author : Sasikumar Dhanarasu
Publisher : BoD – Books on Demand
ISBN 13 : 9535103571
Total Pages : 214 pages
Book Rating : 4.78/5 ( download)

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Download or read book Chromatography and Its Applications written by Sasikumar Dhanarasu and published by BoD – Books on Demand. This book was released on 2012-03-16 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography is a powerful separation tool that is used in all branches of science, and is often the only means of separating components from complex mixtures. The Russian botanist Mikhail Tswett coined the term chromatography in 1906. The first analytical use of chromatography was described by James and Martin in 1952, for the use of gas chromatography for the analysis of fatty acid mixtures. A wide range of chromatographic procedures makes use of differences in size, binding affinities, charge, and other properties. Many types of chromatography have been developed. These include Column chromatography, High performance liquid chromatography (HPLC), Gas chromatography, Size exclusion chromatography, Ion exchange chromatography etc. In this book contains more details about the applications of chromatography by various research findings. Each and every topics of this book have included lists of references at the end to provide students and researchers with starting points for independent chromatography explorations. I welcome comments, criticisms, and suggestions from students, faculty and researchers.

Author : Phyllis R. Brown
Publisher : CRC Press
ISBN 13 : 1000146545
Total Pages : 424 pages
Book Rating : 4.47/5 ( download)

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Download or read book HPLC in Nucleic Acid Research written by Phyllis R. Brown and published by CRC Press. This book was released on 2020-08-19 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives the pertinent information on the high pressure liquid chromatography (HPLC) analyses of all the compounds of interest in nucleic acid metabolism. It aids chromatographers, biochemists, biomedical researchers, and chemists by giving information on applications of HPLC technique.