Apoptosis Methods And Protocols

Author : Hugh J. M. Brady
Publisher : Springer Science & Business Media
ISBN 13 : 1592598129
Total Pages : 306 pages
Book Rating : 4.20/5 ( download)

DOWNLOAD NOW!

Download or read book Apoptosis Methods and Protocols written by Hugh J. M. Brady and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most fundamental question facing each and every cell within an org- ism is to survive or to die. Cell death is required for normal function; some estimates suggest that as many as one million cells undergo cell death every second in the adult human body. Almost all cells undergoing physiological, or programmed, cell death, independent of cell type, manifest a stereotypic p- tern of morphological changes termed apoptosis. Typically, apoptotic cells d- play shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. The integrity of the cell membrane is not lost during apoptosis and so avoids eliciting the inflammatory response that would have been caused by the spillage of the cell’s contents. This is quite in contrast to the loss of cell contents typical of necrosis. The caspases, the family of intracellular cysteine proteases associated with apoptosis, are responsible for the stereotypical m- phological changes. Caspases cleave various substrate proteins that act on DNA fragmentation, nuclear envelope integrity, the cytoskeleton, and cell volume regulation. Apoptotic cells are cleared in vivo by the process of phagocytosis, in which specific “phagocytes” move to the site of apoptosis, engulf the dying cells and digest them. Apoptosis has a central role in many physiological processes, for example, in the immune system. Autoreactive cells are deleted via apoptosis to prevent autoimmunity. At the end of an immune response, activated lymphocytes are removed to maintain homeostasis within the immune system.

Author : P. Erhard
Publisher : Humana Press
ISBN 13 : 9781603270168
Total Pages : 400 pages
Book Rating : 4.67/5 ( download)

DOWNLOAD NOW!

Download or read book Apoptosis written by P. Erhard and published by Humana Press. This book was released on 2009-07-15 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.

Author : Gil Mor
Publisher : Springer Science & Business Media
ISBN 13 : 1588294579
Total Pages : 271 pages
Book Rating : 4.79/5 ( download)

DOWNLOAD NOW!

Download or read book Apoptosis and Cancer written by Gil Mor and published by Springer Science & Business Media. This book was released on 2007-10-11 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.

Author : Hamsa Puthalakath
Publisher : Methods in Molecular Biology
ISBN 13 : 9781493980840
Total Pages : 304 pages
Book Rating : 4.4X/5 ( download)

DOWNLOAD NOW!

Download or read book Programmed Cell Death: Methods and Protocols written by Hamsa Puthalakath and published by Methods in Molecular Biology. This book was released on 2018-05-07 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : P. Erhard
Publisher : Humana Press
ISBN 13 : 9781603270168
Total Pages : 400 pages
Book Rating : 4.67/5 ( download)

DOWNLOAD NOW!

Download or read book Apoptosis written by P. Erhard and published by Humana Press. This book was released on 2009-07-15 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.

Author : Myrtle A. Davis
Publisher : Springer Science & Business Media
ISBN 13 : 1592592791
Total Pages : 165 pages
Book Rating : 4.91/5 ( download)

DOWNLOAD NOW!

Download or read book Apoptosis Methods in Pharmacology and Toxicology written by Myrtle A. Davis and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.

Author : George Studzinski
Publisher : OUP Oxford
ISBN 13 : 0191566004
Total Pages : 267 pages
Book Rating : 4.04/5 ( download)

DOWNLOAD NOW!

Download or read book Apoptosis written by George Studzinski and published by OUP Oxford. This book was released on 1999-12-09 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research.

Author : Ayesha B. Alvero
Publisher : Humana
ISBN 13 : 9781071611647
Total Pages : 265 pages
Book Rating : 4.4X/5 ( download)

DOWNLOAD NOW!

Download or read book Detection of Cell Death Mechanisms written by Ayesha B. Alvero and published by Humana. This book was released on 2021-12-29 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.

Author : Teresa S. Hawley
Publisher : Springer Nature
ISBN 13 : 107163738X
Total Pages : 460 pages
Book Rating : 4.88/5 ( download)

DOWNLOAD NOW!

Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Kimberly McCall
Publisher : Humana Press
ISBN 13 : 9781627033848
Total Pages : 252 pages
Book Rating : 4.4X/5 ( download)

DOWNLOAD NOW!

Download or read book Necrosis written by Kimberly McCall and published by Humana Press. This book was released on 2013-06-04 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Necrosis: Methods and Protocols describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.