Animal Cell Biotechnology

Author : Ralf Pörtner
Publisher : Humana
ISBN 13 : 9781493963188
Total Pages : 0 pages
Book Rating : 4.8X/5 ( download)

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Download or read book Animal Cell Biotechnology written by Ralf Pörtner and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Author : Ralf Pörtner
Publisher : Springer Science & Business Media
ISBN 13 : 1588296601
Total Pages : 1035 pages
Book Rating : 4.03/5 ( download)

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Download or read book Animal Cell Biotechnology written by Ralf Pörtner and published by Springer Science & Business Media. This book was released on 2007-04-05 with total page 1035 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

Author : M. K. Sateesh
Publisher : New Age International
ISBN 13 : 9788122414516
Total Pages : 214 pages
Book Rating : 4.16/5 ( download)

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Download or read book Biotechnology-5: Animal Cells, Immunology & Plant Biotechnology written by M. K. Sateesh and published by New Age International. This book was released on 2003 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Book Is Designed As Per The Syllabus Of Biotechnology Paper-5 Prescribed By Bangalore University And Other Indian Universities. The Book Is Divided Into Three Parts As Follows: * Animal Cell Biotechnology * Immunology * Plant BiotechnologyThe Presentation In Each Part Is Simple And Systematic. The Basic Concepts Have Been Clearly Explained And Their Functions Are Adequately Highlighted. A Few Recent Developments Have Also Been Included To Provide A Contemporary Understanding Of The Subject.

Author : Ashish S. Verma
Publisher : Academic Press
ISBN 13 : 0123914345
Total Pages : 685 pages
Book Rating : 4.47/5 ( download)

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Download or read book Animal Biotechnology written by Ashish S. Verma and published by Academic Press. This book was released on 2013-11-04 with total page 685 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Biotechnology introduces applications of animal biotechnology and implications for human health and welfare. It begins with an introduction to animal cell cultures and genome sequencing analysis and provides readers with a review of available cell and molecular tools. Topics here include the use of transgenic animal models, tissue engineering, nanobiotechnology, and proteomics. The book then delivers in-depth examples of applications in human health and prospects for the future, including cytogenetics and molecular genetics, xenografts, and treatment of HIV and cancers. All this is complemented by a discussion of the ethical and safety considerations in the field.Animal biotechnology is a broad field encompassing the polarities of fundamental and applied research, including molecular modeling, gene manipulation, development of diagnostics and vaccines, and manipulation of tissue. Given the tools that are currently available and the translational potential for these studies, animal biotechnology has become one of the most essential subjects for those studying life sciences. Highlights the latest biomedical applications of genetically modified and cloned animals with a focus on cancer and infectious diseases Provides firsthand accounts of the use of biotechnology tools, including molecular markers, stem cells, and tissue engineering

Author :
Publisher : Elsevier
ISBN 13 : 0080925995
Total Pages : 409 pages
Book Rating : 4.98/5 ( download)

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Download or read book Animal Cell Biotechnology written by and published by Elsevier. This book was released on 2012-12-02 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: There has been a dramatic increase in the perception of the value of animal cell biotechnology to the research and manufacturing communities in recent years. This volume seeks to keep the reader up-to-date with this progress. This sixth and final volume in the series concentrates on the biology of animal cells in culture, giving special attention to the relationship between biology and the ability to use such cells productively. As the search continues for greater productivity, there is a need to understand the switches within cells that control expression. Additional abilities to manipulate those switches in a controllable manner are also required. In the last five years, considerable progress has been made in the elucidation of the mechanisms for cell signaling and control of gene expression. The 13 chapters of this volume are devoted to these subjects and to techniques in areas of particular concern in manufacturing circles. The achievements in the field to date are described in this book, which, together with its five companion volumes in the series, will provide a building block for the future development of animal cell biotechnology.

Author : Hansjörg Hauser
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110381427
Total Pages : 715 pages
Book Rating : 4.29/5 ( download)

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Download or read book Animal Cell Biotechnology written by Hansjörg Hauser and published by Walter de Gruyter GmbH & Co KG. This book was released on 2014-11-10 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Author : Nigel Jenkins
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.39/5 ( download)

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Download or read book Animal Cell Biotechnology written by Nigel Jenkins and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309084393
Total Pages : 201 pages
Book Rating : 4.90/5 ( download)

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Download or read book Animal Biotechnology written by National Research Council and published by National Academies Press. This book was released on 2002-12-29 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.

Author : R. E. Spier
Publisher : Butterworth-Heinemann
ISBN 13 : 1483165159
Total Pages : 853 pages
Book Rating : 4.58/5 ( download)

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Download or read book Production of Biologicals from Animal Cells in Culture written by R. E. Spier and published by Butterworth-Heinemann. This book was released on 2013-09-24 with total page 853 pages. Available in PDF, EPUB and Kindle. Book excerpt: Production of Biologicals from Animal Cells in Culture reviews the state of the art in animal cell biotechnology, with emphasis on the sequence of events that occur when generating a biological from animal cells in culture. Methods that enable adjustment of nutrient feed streams into perfusion bioreactors so as to increase productivity are described. A number of issues are also addressed, such as the usefulness of the fingerprint method for cell characterization. Comprised of 135 chapters, this book begins with an overview of the problems and benefits of animal cell culture, followed by a discussion on the isolation of immortal murine macrophage cell lines. The reader is systematically introduced to the use of DNA fingerprinting to characterize cell banks; immortalization of cells with oncogenes; lipid metabolism of animal cells in culture; and energetics of glutaminolysis. Subsequent chapters explore serum-free and protein-free media; the physiology of animal cells; gene expression in animal cell systems; and animal cell bioreactors. The monitoring and assay of animal cell parameters are also considered, along with downstream processing and regulatory issues. This monograph will be of interest to students, practitioners, and investigators in the fields of microbiology and biotechnology.

Author : Michael Butler
Publisher : Taylor & Francis
ISBN 13 : 1135325383
Total Pages : 299 pages
Book Rating : 4.81/5 ( download)

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Download or read book Animal Cell Culture and Technology written by Michael Butler and published by Taylor & Francis. This book was released on 2004-08-02 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides all essential practical information for establishing a laboratory animal cell culture. Comprehensive glossary of terms.