A Laboratory Guide For Cellular And Molecular Plant Biology

Author : I. Negrutiu
Publisher : Birkhäuser
ISBN 13 : 3034875029
Total Pages : 383 pages
Book Rating : 4.28/5 ( download)

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Download or read book A Laboratory Guide for Cellular and Molecular Plant Biology written by I. Negrutiu and published by Birkhäuser. This book was released on 2013-03-09 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.

Author : I. Negrutiu
Publisher : Birkhäuser
ISBN 13 : 9783764325428
Total Pages : 388 pages
Book Rating : 4.29/5 ( download)

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Download or read book A Laboratory Guide for Cellular and Molecular Plant Biology written by I. Negrutiu and published by Birkhäuser. This book was released on 1991-11-01 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.

Author : Melody S. Clark
Publisher : Springer Science & Business Media
ISBN 13 : 3642878733
Total Pages : 551 pages
Book Rating : 4.32/5 ( download)

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Download or read book Plant Molecular Biology — A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.

Author : M. Clark
Publisher :
ISBN 13 : 9780387584058
Total Pages : pages
Book Rating : 4.56/5 ( download)

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Download or read book Plant molecular biology: a laboratory manual written by M. Clark and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : M.S. Punia
Publisher : Scientific Publishers
ISBN 13 : 9388172361
Total Pages : 255 pages
Book Rating : 4.63/5 ( download)

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Download or read book Plant Biotechnology and Molecular Biology : A Laboratory Manual written by M.S. Punia and published by Scientific Publishers. This book was released on 2018-01-01 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols.

Author : Heather B. Miller
Publisher : Academic Press
ISBN 13 : 0123855454
Total Pages : 227 pages
Book Rating : 4.59/5 ( download)

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Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Russell Malmberg
Publisher : CSHL Press
ISBN 13 : 9780879691844
Total Pages : 162 pages
Book Rating : 4.40/5 ( download)

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Download or read book Molecular Biology of Plants written by Russell Malmberg and published by CSHL Press. This book was released on 1985 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : John Davey
Publisher : OUP Oxford
ISBN 13 : 9780199638307
Total Pages : 424 pages
Book Rating : 4.06/5 ( download)

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Download or read book Essential Cell Biology Vol 1 written by John Davey and published by OUP Oxford. This book was released on 2003-06-12 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in our understanding of cells has put cell biology at the center of biological and medical research. This two volume set provides researchers with the information they need to understand and carry out the essential techniques used for studying cells. It covers a wide range of traditional and recently developed techniques and includes the fine detail necessary for immediate application in the laboratory. It is useful both as a compendium of protocols for experienced researchers and as a valuable guide for newcomers to the subject.

Author : R. Malmberg
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.84/5 ( download)

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Download or read book Molecular Biology of Plants written by R. Malmberg and published by . This book was released on 1985 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : P. Maliga
Publisher :
ISBN 13 :
Total Pages : 478 pages
Book Rating : 4.26/5 ( download)

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Download or read book Methods in Plant Molecular Biology written by P. Maliga and published by . This book was released on 1995 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: