3d Stem Cell Culture

Author : Joni H. Ylostalo
Publisher : MDPI
ISBN 13 : 3039438034
Total Pages : 186 pages
Book Rating : 4.37/5 ( download)

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Download or read book 3D Stem Cell Culture written by Joni H. Ylostalo and published by MDPI. This book was released on 2021-01-07 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.

Author : Zuzana Koledova
Publisher : Humana Press
ISBN 13 : 9781493970193
Total Pages : 452 pages
Book Rating : 4.94/5 ( download)

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Download or read book 3D Cell Culture written by Zuzana Koledova and published by Humana Press. This book was released on 2017-06-21 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.

Author : Joni H. Ylostalo
Publisher :
ISBN 13 : 9783039438044
Total Pages : 186 pages
Book Rating : 4.42/5 ( download)

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Download or read book 3D Stem Cell Culture written by Joni H. Ylostalo and published by . This book was released on 2021 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.

Author : Stefan Przyborski
Publisher : John Wiley & Sons
ISBN 13 : 1118851501
Total Pages : 424 pages
Book Rating : 4.00/5 ( download)

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Download or read book Technology Platforms for 3D Cell Culture written by Stefan Przyborski and published by John Wiley & Sons. This book was released on 2017-05-01 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture Features contributions from leading developers and researchers active in 3D cell technology Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied Includes information on resources and technical support to help initiate the use of 3D culture methods

Author : John Haycock
Publisher : Humana Press
ISBN 13 : 9781607619833
Total Pages : 0 pages
Book Rating : 4.30/5 ( download)

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Download or read book 3D Cell Culture written by John Haycock and published by Humana Press. This book was released on 2010-11-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.

Author : Cornelia Kasper
Publisher : Springer Nature
ISBN 13 : 3030667499
Total Pages : 252 pages
Book Rating : 4.98/5 ( download)

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Download or read book Basic Concepts on 3D Cell Culture written by Cornelia Kasper and published by Springer Nature. This book was released on 2021-06-09 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook shall introduce the students to 3D cell culture approaches and applications. An overview on existing techniques and equipment is provided and insight into various aspects and challenges that researchers need to consider and face during culture of 3D cells is given. The reader will learn the importance of physiological cell, tissue and organ models and gains important knowledge on 3D analytics. This textbook deepens selected aspects of the textbook “Cell Culture Technology”, which also is published in this series, while offering extended insight into 3D cell culture. The concept of the textbook encompasses various lectures ranging from basics in cell cultivation, tissue engineering, biomaterials and biocompatibility, in vitro test systems and regenerative medicine. The textbook addresses Master- and PhD students interested and/or working in the field of modern cell culture applications and will support the understanding of the essential strategies in 3D cell culture and waken awareness for the potentials and challenges of this application.

Author : Royce Fortin
Publisher : States Academic Press
ISBN 13 : 9781639897940
Total Pages : 0 pages
Book Rating : 4.41/5 ( download)

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Download or read book 3D Stem Cell Culture: Stem Cell Biology Essentials written by Royce Fortin and published by States Academic Press. This book was released on 2023-09-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A 3D stem cell culture is an artificially created environment that allows biological cells to grow or interact with surrounding extracellular environment in three dimensions. It can be classified into two types of methods, namely, scaffold techniques and scaffold-free techniques. Solid scaffolds, hydrogels, and other materials are used in scaffold techniques. Recent studies have explored the potentiality of human CD34+ stem cells generated in-vitro agarose gel 3D model to understand the process of bone ossification. Scaffold-free techniques include the use of low adhesion plates, hanging drop plates, micropatterned surfaces, rotating bioreactors, magnetic levitation, and magnetic 3D bioprinting. This book presents recent researches on 3D stem cell culture. Scientists and students actively engaged in the field of stem cell biology will find it full of crucial and unexplored concepts.

Author : Zuzana Sumbalova Koledova
Publisher : Springer Nature
ISBN 13 : 107163674X
Total Pages : 337 pages
Book Rating : 4.49/5 ( download)

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Download or read book 3D Cell Culture written by Zuzana Sumbalova Koledova and published by Springer Nature. This book was released on with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Ranjna C. Dutta
Publisher : CRC Press
ISBN 13 : 1351378511
Total Pages : 212 pages
Book Rating : 4.12/5 ( download)

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Download or read book 3D Cell Culture written by Ranjna C. Dutta and published by CRC Press. This book was released on 2018-05-11 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D cell culture is yet to be adopted and exploited to its full potential. It promises to upgrade and bring our understanding about human physiology to the highest level with the scope of applying the knowledge for better diagnosis as well as therapeutics. The focus of this book is on the direct impact of novel technologies and their evolution into viable products for the benefit of human race. It also describes the fundamentals of cell microenvironment to bring forth the relevance of 3D cell culture in tissue engineering and regenerative medicine. It discusses the extracellular matrix/microenvironment (ECM) and emphasizes its significance for growing cells in 3D to accomplish physiologically viable cell mass/tissue ex vivo. The book bridges the knowledge gaps between medical need and the technological applications through illustrations. It discusses the available models for 3D cell culture as well as the techniques to create substrates and scaffolds for achieving desired 3D microenvironment.

Author : Takashi Tsuji
Publisher :
ISBN 13 : 9781493969494
Total Pages : 248 pages
Book Rating : 4.98/5 ( download)

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Download or read book Organ Regeneration written by Takashi Tsuji and published by . This book was released on 2017 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.